Influence of Particle Size on Persistence and Clearance of Aerosolized Silver Nanoparticles in the Rat Lung
Influence of Particle Size on Persistence and Clearance of Aerosolized Silver Nanoparticles in the Rat Lung
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DOI：
10.1093/toxsci/kfv005
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发表时间：
2015-04-01
影响因子：
3.8
通讯作者：
Van Winkle, Laura S.
中科院分区：
文献类型：
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作者：
Anderson, Donald S.;Patchin, Esther S.;Van Winkle, Laura S.
消费品中越来越多地使用纳米银颗粒 (AgNP)，引发了人们对潜在健康影响的担忧。本研究调查了通过单次仅鼻气溶胶暴露（分别为 7.2 和 5.4 mg/m(3)）（6 小时）向大鼠递送的 2 种不同尺寸的 AgNP（20 和 110 nm）的持久性和清除率。使用电感耦合等离子体质谱 (ICP-MS)、自动金相术和增强暗场显微镜评估大鼠肺组织的银积累情况。通过支气管肺泡灌洗液（BALF）中银染色的评分来评估组织巨噬细胞的参与。暴露后 1 天，大多数巨噬细胞中银含量丰富。暴露于 20 nm AgNP 的组在暴露后 56 天时具有最多数量的银阳性 BALF 巨噬细胞。虽然与暴露后 1 天相比，暴露后 56 天肺组织中的银量显着减少，但两种 AgNP 仍存在至少 33% 的初始递送剂量。无论颗粒大小如何，银主要位于与细胞外基质相关的肺终末支气管/肺泡管连接区域内以及上皮细胞内。吸入 20 和 110 nm AgNP 会导致暴露后 56 天的银在肺部持续存在，并导致银在终末细支气管肺泡管连接处局部沉积和积聚。此外，较小的颗粒（20 nm AgNP）在 BALF 巨噬细胞中产生更大的银负荷，并且在稍后的时间点（21 和 56 天）银阳性巨噬细胞的持久性更强。
The growing use of silver nanoparticles (AgNPs) in consumer products raises concerns about potential health effects. This study investigated the persistence and clearance of 2 different size AgNPs (20 and 110 nm) delivered to rats by single nose-only aerosol exposures (6 h) of 7.2 and 5.4 mg/m(3), respectively. Rat lung tissue was assessed for silver accumulations using inductively-coupled plasma mass spectrometry (ICP-MS), autometallography, and enhanced dark field microscopy. Involvement of tissue macrophages was assessed by scoring of silver staining in bronchoalveolar lavage fluid (BALF). Silver was abundant in most macrophages at 1 day post-exposure. The group exposed to 20 nm AgNP had the greatest number of silver positive BALF macrophages at 56 days post-exposure. While there was a significant decrease in the amount of silver in lung tissue at 56 days post-exposure compared with 1 day following exposure, at least 33% of the initial delivered dose was still present for both AgNPs. Regardless of particle size, silver was predominantly localized within the terminal bronchial/alveolar duct junction region of the lung associated with extracellular matrix and within epithelial cells. Inhalation of both 20 and 110 nm AgNPs resulted in a persistence of silver in the lung at 56 days post-exposure and local deposition as well as accumulation of silver at the terminal bronchiole alveolar duct junction. Further the smaller particles, 20 nm AgNP, produced a greater silver burden in BALF macrophages as well as greater persistence of silver positive macrophages at later timepoints (21 and 56 days).