Quantitative trait loci for locomotor behavior in Drosophila melanogaster
Quantitative trait loci for locomotor behavior in Drosophila melanogaster
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DOI：
10.1534/genetics.106.058099
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发表时间：
2006-09-01
期刊：
影响因子：
3.3
通讯作者：
Mackay, Trudy F. C.
中科院分区：
文献类型：
--
作者：
Jordan, Katherine W.;Morgan, Theodore J.;Mackay, Trudy F. C.
运动是大多数动物行为的组成部分，许多人类疾病和障碍与运动缺陷有关，但对运动行为自然变异的遗传基础知之甚少。运动是一个复杂的性状，其变异归因于多个相互作用的数量性状基因座（QTL）的联合分离，其效应对环境敏感。我们评估了一个组成部分的运动行为（运动反应性）在人口中的98个重组近交系的果蝇和映射4个QTL影响运动反应性连锁多态性太转座因子插入位点的变化。我们使用缺陷的互补测试来精细地将这些QTL定位到12个染色体区域，并使用突变的互补测试来确定13个影响运动反应性的位置候选基因，包括多巴脱羧酶（Ddc），它催化5-羟色胺和多巴胺合成的最后一步。来自一个单一的自然群体的164个第二染色体置换系的人口中的连锁不平衡映射表明，Ddc的多态性与自然发生的运动行为的遗传变异。这些数据暗示生物胺合成的变化是导致运动反应性自然变化的一个因素。
Locomotion is an integral component of most animal behaviors and many human diseases and disorders are associated with locomotor deficits, but little is known about the genetic basis of natural variation in locomotor behavior. Locomotion is a complex trait, with variation attributable to the joint segregation of multiple interacting quantitative trait loci (QTL), with effects that are sensitive to the environment. We assessed variation in a component of locomotor behavior (locomotor reactivity) in a population of 98 recombinant inbred lines of Drosophila melanogaster and mapped four QTL affecting locomotor reactivity by linkage to polymorphic Too transposable element insertion sites. We used complementation tests of deficiencies to fine map these QTL to 12 chromosomal regions and complementation tests of mutations to identify 13 positional candidate genes affecting locomotor reactivity, including Dopa decarboxylase (Ddc), which catalyzes the final step in the synthesis of serotonin and dopamine. Linkage disequilibrium mapping in a population of 164 second chromosome substitution lines derived from a single natural population showed that polymorphisms at Ddc were associated with naturally occurring genetic variation in locomotor behavior. These data implicate variation in the synthesis of bioamines as a factor contributing to natural variation in locomotor reactivity.