Recent Advances in the Understanding of Teleost Medaka Ovulation: The Roles of Proteases and Prostaglandins
Recent Advances in the Understanding of Teleost Medaka Ovulation: The Roles of Proteases and Prostaglandins
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DOI：
10.2108/zsj.30.239
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发表时间：
2013-03
期刊：
影响因子：
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通讯作者：
Takayuki Takahashi;Chika Fujimori;Akane Hagiwara;K. Ogiwara
中科院分区：
文献类型：
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作者：
Takayuki Takahashi;Chika Fujimori;Akane Hagiwara;K. Ogiwara
排卵是从排卵前卵泡中释放卵母细胞的过程，几乎在所有雌性脊椎动物的卵巢中都可以观察到。与哺乳动物物种相比，关于非哺乳动物物种排卵机制的研究要少得多。我们已经研究了卵泡破裂的分子机制，在排卵期使用硬骨鱼模型，青，或青。青鳉排卵中卵泡破裂涉及金属蛋白酶组织抑制剂-2b蛋白与至少三种基质金属蛋白酶（MMP）的合作：膜型-1 MMP（MT 1-MMP）、MT 2-MMP和明胶酶A。我们的研究还表明，丝氨酸蛋白酶，即，纤溶酶在排卵时MMP介导的水解激活之前仅参与破裂数小时。前列腺素E2（PGE 2）在青鳉排卵的参与也被证明。环氧合酶-2和PGE 2受体亚型EP 4 b分别被证明是负责PGE 2合成的酶和排卵前卵泡中产生的配体的受体。根据我们对鱼类的研究结果，我们讨论了脊椎动物排卵与哺乳动物排卵的相似性和差异。
Ovulation is the process of liberating oocytes from the preovulatory follicles, and is observed in the ovaries of virtually all female vertebrate animals. Compared with mammalian species, there have been far fewer studies that address the ovulatory mechanisms of non-mammalian species. We have examined the molecular mechanism of follicle rupture during ovulation using the teleost model, medaka, or Oryzias latipes. Follicle rupture in medaka ovulation involves the cooperation of the tissue inhibitor of metalloproteinase-2b protein with at least three matrix metalloproteinases (MMP): membrane type-1 MMP (MT1-MMP), MT2-MMP, and gelatinase A. Our studies also indicate that the serine protease, i.e., plasmin, participates in the rupture for only a few hours prior to the activation of MMP-mediated hydrolysis at ovulation. The involvement of prostaglandin E2 (PGE2) in medaka ovulation was also demonstrated. Cyclooxygenase-2 and PGE2 receptor subtype EP4b were respectively shown to be an enzyme responsible for PGE2 synthesis and a receptor for the generated ligand in the preovulatory follicles. Based on the results obtained from our studies of fish, we discuss the similarities and differences in vertebrate ovulation compared with mammalian species.