Sequence-specific recognition of DNA. Nuclear magnetic resonance assignments and structural comparison of wild-type and mutant lambda OR3 operator DNA.
Sequence-specific recognition of DNA. Nuclear magnetic resonance assignments and structural comparison of wild-type and mutant lambda OR3 operator DNA.
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DNA 的序列特异性识别。
DOI：
10.1016/0022-2836(84)90429-7
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发表时间：
1984
影响因子：
5.6
通讯作者：
Reid,BR
中科院分区：
文献类型：
--
作者：
Wemmer,DE;Chou,SH;Reid,BR
噬菌体 λ 的 17 个碱基对 OR3 操纵子和 vC3 单碱基对突变体的两条链中所有碱基质子和大部分糖质子的共振，已使用二维核磁共振方法进行了分配。这两个 DNA 序列的化学位移和核奥沃豪塞效应数据表明，突变远端位点没有结构扰动，突变序列中改变的碱基对周围的结构也没有显着变化。这些结果与 Ohlendorfet al.(1982) 提出的模型一致，该模型基于 cro 蛋白的晶体学数据，用于 OR3-cro 蛋白相互作用。根据该模型对这些溶液研究的数据进行了检查和讨论。这项工作表明，核磁共振化学位移和核奥沃豪瑟效应强度提供了一种比较 DNA 分子溶液结构的方法。从所研究的 17 个碱基对操作子的光谱中可用的分辨率来看，很明显，可以使用相敏方法研究多达 30 个或更多碱基对的 DNA 双链体。
The resonances of all the base protons and most of the sugar protons in both strands of the 17 base-pair OR3 operator of the phage λ, and of the vC3 single base-pair mutant, have been assigned using two-dimensional nuclear magnetic resonance methods. The chemical shift and nuclear Overhauser effect data for these two DNA sequences reveal no structural perturbation at sites distal to the mutation, neither are there significant changes in structure immediately surrounding the altered base-pair in the mutant sequence. These results are consistent with the model proposed by Ohlendorfet al.(1982), based on crystallographic data on the cro protein, for the OR3-cro protein interaction. The data from these solution studies are examined and discussed in the light of this model.This work demonstrates that nuclear magnetic resonance chemical shifts and nuclear Overhauser effect intensities provide a method for comparing the solution structures of DNA molecules. From the resolution available in the spectra of the 17 base-pair operators studied, it is clear that DNA duplexes of up to 30 or more base-pairs can be studied using phase-sensitive methods.