Monocyte recruitment to endothelial cells in response to oscillatory shear stress
Monocyte recruitment to endothelial cells in response to oscillatory shear stress
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DOI：
10.1096/fj.02-1064com
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发表时间：
2003-09-01
期刊：
影响因子：
4.8
通讯作者：
Ho, CM
中科院分区：
文献类型：
--
作者：
Hsiai, TK;Cho, SK;Ho, CM
白细胞募集至内皮细胞是炎症反应中的关键事件。迄今为止，这些反应背后的剪切应力、拓扑结构和细胞相互作用的结果的空间、时间梯度都是从组织切片的静态成像或静态培养细胞的研究中推断出来的。在本报告中，我们开发了微机电系统（MEMS）传感器，其尺寸与单个内皮细胞（EC）相当，可将复杂流动环境中的实时剪切应力与单核细胞/EC结合动力学联系起来，以高空间和时间分辨率模拟动脉分叉处的移动和不稳定分离点。响应于时间平均剪切应力 (tau(ave)) = 0 和 0.5 Hz +/- 2.6 dyn/cm(2) 的振荡剪切应力 (tau)，单个单核细胞表现出独特的往复轨迹，与其他单核细胞进行滚动、结合和解离，然后在 EC 上牢固粘附。我们的研究根据位移和速度曲线量化了单个单核细胞/EC 结合动力学。振荡流诱导粘附分子和细胞因子的上调，以在剪切应力动态范围+/- 2.6 dyn/cm(2) 内介导单核细胞/EC 相互作用（P = 0.50，n = 10）。
Leukocyte recruitment to endothelial cells is a critical event in inflammatory responses. The spatial, temporal gradients of shear stress, topology, and outcome of cellular interactions that underlie these responses have so far been inferred from static imaging of tissue sections or studies of statically cultured cells. In this report, we developed micro-electromechanical systems (MEMS) sensors, comparable to a single endothelial cell (EC) in size, to link real-time shear stress with monocyte/EC binding kinetics in a complex flow environment, simulating the moving and unsteady separation point at the arterial bifurcation with high spatial and temporal resolution. In response to oscillatory shear stress (tau) at +/- 2.6 dyn/cm(2) at a time-averaged shear stress (tau(ave)) = 0 and 0.5 Hz, individual monocytes displayed unique to-and-fro trajectories undergoing rolling, binding, and dissociation with other monocyte, followed by solid adhesion on EC. Our study quantified individual monocyte/EC binding kinetics in terms of displacement and velocity profiles. Oscillatory flow induces up-regulation of adhesion molecules and cytokines to mediate monocyte/EC interactions over a dynamic range of shear stress +/- 2.6 dyn/cm(2) (P = 0.50, n = 10).