Coculture Fermentations of Bifidobacterium Species and Bacteroides thetaiotaomicron Reveal a Mechanistic Insight into the Prebiotic Effect of Inulin-Type Fructans
Coculture Fermentations of Bifidobacterium Species and Bacteroides thetaiotaomicron Reveal a Mechanistic Insight into the Prebiotic Effect of Inulin-Type Fructans
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DOI：
10.1128/aem.02649-08
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发表时间：
2009-04-01
影响因子：
4.4
通讯作者：
De Vuyst, Luc
中科院分区：
文献类型：
--
作者：
Falony, Gwen;Calmeyn, Thomas;De Vuyst, Luc
四种双歧杆菌，每一种都代表一群具有特定菊糖型果聚糖降解能力的菌株，与拟杆菌thetaiotaomicron LMG 11262共培养发酵，该菌株能够代谢低聚果糖和菊糖。在以菊粉作为唯一补充能量来源的结肠细菌培养基中，双歧杆菌竞争这种底物的能力反映了表型差异。短双歧杆菌养乐多是一种不能降解低聚果糖或菊糖的菌株，被b.s thetaiotaommicron LMG 11262打败。青少年双歧杆菌LMG 10734是一种可以降解低聚果糖的菌株（表现出优先分解机制），但不能在菊粉上生长，当低聚果糖和菊粉的短片段开始在发酵培养基中积累时，它成功地变得具有竞争力。角双歧杆菌LMG 11039(T)，一种以前被证明可以同时降解所有低聚果糖组分并能够部分分解菊粉的菌株，从发酵开始就具有竞争力，从菊粉出现的那一刻起就消耗短片段。长双歧杆菌LMG 11047是与双歧杆菌LMG 11262共培养的优势菌株，它具有高果糖消耗和低聚果糖降解率，并能够部分分解菊粉。这些观察结果表明，大肠双歧杆菌种群中的不同亚群将受到不同群的益生元胰岛素型果聚糖的刺激，这种差异可能反映在它们促进健康作用的差异上。
Four bifidobacteria, each representing a cluster of strains with specific inulin-type-fructan degradation capacities, were grown in coculture fermentations with Bacteroides thetaiotaomicron LMG 11262, a strain able to metabolize both oligofructose and inulin. In a medium for colon bacteria with inulin as the sole added energy source, the ability of the bifidobacteria to compete for this substrate reflected phenotypical variation. Bifidobacterium breve Yakult, a strain that was not able to degrade oligofructose or inulin, was outcompeted by B. thetaiotaomicron LMG 11262. Bifidobacterium adolescentis LMG 10734, a strain that could degrade oligofructose (displaying a preferential breakdown mechanism) but that did not grow on inulin, managed to become competitive when oligofructose and short fractions of inulin started to accumulate in the fermentation medium. Bifidobacterium angulatum LMG 11039(T), a strain that was previously shown to degrade all oligofructose fractions simultaneously and to be able to partially break down inulin, was competitive from the beginning of the fermentation, consuming short fractions of inulin from the moment they appeared. Bifidobacterium longum LMG 11047, representing a cluster of bifidobacteria that shared both high fructose consumption and oligofructose degradation rates and were able to perform partial breakdown of inulin, was the dominating strain in a coculture with B. thetaiotaomicron LMG 11262. These observations indicate that distinct subgroups within the large-intestinal Bifidobacterium population will be stimulated by different groups of prebiotic inulin-type fructans, a variation that could be reflected in differences concerning their health-promoting effects.