Orchestration of plant development and defense by indirect crosstalk of salicylic acid and brassinosteorid signaling via transcription factor GhTINY2
Orchestration of plant development and defense by indirect crosstalk of salicylic acid and brassinosteorid signaling via transcription factor GhTINY2
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通过转录因子 GhTINY2 水杨酸和油菜素甾类信号传导的间接串扰来协调植物发育和防御
DOI：
10.1093/jxb/erab186
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发表时间：
2021
影响因子：
6.9
通讯作者：
Zhu Longfu
中科院分区：
文献类型：
--
作者：
Xiao Shenghua;Hu Qin;Zhang Xiaojun;Si Huan;Liu Shiming;Chen Lin;Chen Kun;Berne Sabina;Yuan Daojun;Lindsey Keith;Zhang Xianlong;Zhu Longfu
水杨酸(SA)和油菜素类固醇(BRS)调节植物发育和逆境反应的不同过程是众所周知的，但这些植物激素调节生长和防御权衡的机制在很大程度上还不清楚。此外，对脱水反应元件结合转录因子的作用，特别是在生物胁迫和植物生长中的作用，人们知之甚少。在此，我们鉴定了一个棉花(Gossypium Hirsutom)APETALA2/乙烯反应因子基因GhTINY2，该基因受黄萎病的强烈诱导。GhTINY2在棉花和拟南芥中的过表达提高了对小麦的耐性。而表达下调则增加了棉花对该病原菌的感受性。GhTINY2通过直接激活WRKY51的表达来促进SA的积累和SA信号转导。此外，过量表达GhTINY2的棉花和拟南芥表现出生长迟缓，对BR生物合成抑制剂的敏感性增加，几个BR诱导基因下调，BR抑制基因上调，而GhTINY2-RNAi棉花则表现出相反的效果。我们进一步确定GhTINY2通过与BRASSINAZOLE抗性1(BZR1)相互作用并抑制其对吲哚-3-乙酸诱导的19(IAA19)表达的转录激活而负调控BR信号。这些发现表明，GhTINY2通过WRKY51介导的SA生物合成和BZR1-IAA19调节的BR信号之间的间接串扰，微调了免疫和生长之间的权衡。
Salicylic acid (SA) and brassinosteroids (BRs) are well known to regulate diverse processes of plant development and stress responses, but the mechanisms by which these phytohormones mediate the growth and defense trade-off are largely unclear. In addition, little is known about the roles of DEHYDRATION RESPONSIVE ELEMENT BINDING transcription factors, especially in biotic stress and plant growth. Here, we identified a cotton (Gossypium hirsutum) APETALA2/ETHYLENE RESPONSIVE FACTOR geneGhTINY2that is strongly induced byVerticillium dahliae. Overexpression ofGhTINY2in cotton and Arabidopsis enhanced tolerance toV. dahliae, while knockdown of expression increased the susceptibility of cotton to the pathogen. GhTINY2 was found to promote SA accumulation and SA signaling transduction by directly activating expression ofWRKY51. Moreover,GhTINY2-overexpressing cotton and Arabidopsis showed retardation of growth, increased sensitivity to inhibitors of BR biosynthesis, down-regulation of several BR-induced genes, and up-regulation of BR-repressed genes, whileGhTINY2-RNAi cotton showed the opposite effects. We further determined that GhTINY2 negatively regulates BR signaling by interacting with BRASSINAZOLE-RESISTANT 1 (BZR1) and restraining its transcriptional activation of the expression ofINDOLE-3-ACETIC ACID INDUCIBLE 19(IAA19). These findings indicate that GhTINY2 fine-tunes the trade-off between immunity and growth via indirect crosstalk between WRKY51-mediated SA biosynthesis and BZR1-IAA19-regulated BR signaling.