The release of DNA into the plasma of mice following hepatic cell death by apoptosis and necrosis.
The release of DNA into the plasma of mice following hepatic cell death by apoptosis and necrosis.
复制标题
小鼠肝细胞因凋亡和坏死而死亡后，DNA 释放到血浆中。
DOI：
10.1080/13547500701791719
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Pisetsky,DavidS
中科院分区：
文献类型：
--
作者：
Tran,TrinhT;Groben,Pamela;Pisetsky,DavidS
The goal of these investigations was to measure levels of DNA in the plasma of mice following administration of hepatotoxic agents to induce apoptotic or necrotic cell death and determine any differences in the release of this marker depending upon death pathway. For this purpose, the effects of varying doses of anti-Fas, acetaminophen (APAP) or carbon tetrachloride (CCl4) were assessed in normal mice. Plasma DNA was measured fluorometrically by the dye PicoGreen while lactate dehydrogenase (LDH) and caspase 3, other molecules released with cell injury or death, were measured by enzymatic assays. Histology was used to assess the occurrence of apoptosis or necrosis. Results of these experiments indicate that increased blood DNA levels occurred with all three agents and were highest with anti-Fas and CCl4; caspase 3 levels were much higher with anti-Fas than the other agents. Histological examination confirmed the predominance of apoptotic death with anti-Fas and necrotic death with APAP and CCl4. These results indicate that increased blood DNA is common in hepatotoxic injury and is a feature of both apoptotic and necrotic death.
