THE MICROSTRUCTURAL TENSILE PROPERTIES AND BIOCHEMICAL-COMPOSITION OF THE BOVINE DISTAL FEMORAL GROWTH PLATE
THE MICROSTRUCTURAL TENSILE PROPERTIES AND BIOCHEMICAL-COMPOSITION OF THE BOVINE DISTAL FEMORAL GROWTH PLATE
复制标题
DOI：
10.1002/jor.1100100214
复制
发表时间：
1992-03-01
影响因子：
2.8
通讯作者：
MOW, VC
中科院分区：
文献类型：
--
作者：
COHEN, B;CHORNEY, GS;MOW, VC
在从骺板上预定解剖部位均匀制备的直型骨-生长板-骨样本（横截面为7 x 7 mm 2）上，测定12月龄奶牛股骨远端生长板的拉伸性能。使用计算机控制的伺服液压试验机以0.004 s-1的应变速率将每个样本分散至失效。结果发现，指数本构关系，使用有限变形公式的应变，提供了一个很好的描述的应力-应变行为的所有标本的故障点。极限应力和两个切线模量（即，趾部切线模量和在75%极限应变下计算的切线模量）随解剖部位而变化。前部最强，其次是后部/外侧。前部和后部/外侧区域也是最硬的，而后部/内侧和中心区域是最弱和最柔顺的。骨-生长板-骨样本显示出较低的极限应变（13.8% +/-6%），在整个生长板中没有显著变化。这一结果表明，即使在目前推荐的临床软骨松解术延长腿的较低牵引力下，也可能在体内发生骨骺板的破坏。生长板在解剖区域的生物化学组成与拉伸性能相关。有一个更大的胶原蛋白含量的地区是最硬和最强的。本研究还描述了牛股骨远端生长板的大体形态。生长骨骺板在几个维度上的起伏有一个规则的模式。组织学结果显示肥大细胞柱和经骺间隔的方向几乎平行于骨干的纵轴。该脊柱方向不受生长骨骺的主要轮廓的波动的影响，生长骨骺在某些位置可能相对于骨干轴倾斜多达60度。肥大细胞柱的取向似乎是影响骨生长板-骨标本拉伸行为的主要显微结构特征之一。
The tensile properties of distal femoral growth plates from 12-month-old cows were determined on uniformily prepared straight bone-growth plate-bone specimens (7 x 7 mm2 in cross-section) from predetermined anatomical sites on the physis. Each specimen was distracted to failure using a computer-controlled servo-hydraulic testing machine at a strain rate of 0.004 s-1. It was found that the exponential constitutive law, using finite deformation formulation for strain, provides an excellent description of the stress-strain behavior of all the specimens up to the point of failure. The ultimate stress and both tangent moduli (i.e., the toe region tangent modulus and the tangent modulus calculated at 75% of ultimate strain) varied with anatomical site. The anterior region was the strongest, followed by the posterior/lateral. The anterior and posterior/lateral regions were also the stiffest, whereas the posterior/medial and center regions were the weakest and most compliant. The bone-growth plate-bone specimen exhibited a low ultimate strain (13.8% +/-6%) that did not vary significantly throughout the growth plate. This result suggests that disruption of the physis may occur in vivo even at the lower distractions currently recommended for the clinical chondrodiatasis procedure for leg lengthening. The biochemical composition of the growth plate in the anatomical regions correlated well with the tensile properties. There was a greater collagen content in the regions that were the stiffest and strongest. The gross morphology of the growth plate of the bovine distal femur is also described in this study. There is a regular pattern to the undulations of the physis at several dimensional levels. Histologic findings showed that orientation of the hypertrophic cell columns and transphyseal septa are aligned nearly parallel to the longitudinal axis of the diaphyseal shaft. This column orientation is not affected by the undulation of the primary contour of the physis, which at certain locations may be inclined as much as 60-degrees relative to the diaphyseal axis. The orientation of the hypertrophic cell columns appears to be one of the dominant microstructural features influencing the tensile behavior of the bone-growth plate-bone specimens.