Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures
Orexin decreases mRNA expressions of NMDA and AMPA receptor subunits in rat primary neuron cultures
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DOI：
10.1016/j.peptides.2008.05.002
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发表时间：
2008-09-01
期刊：
影响因子：
3
通讯作者：
Nakao, Kazuwa
中科院分区：
文献类型：
--
作者：
Yamada, Nobuko;Katsuura, Goro;Nakao, Kazuwa
食欲素是下丘脑中产生食欲的神经肽之一。下丘脑外侧 (LH) 中的食欲素神经元投射到大脑皮层和海马体中，受体在其中分布有高浓度。因此，为了阐明食欲素在大脑皮层中的作用，我们在施用食欲素-A或6天后检查了其对N-甲基-D-天冬氨酸（NMDA）受体亚基（NR1、NR2A、NR2B）和α-氨基-3-羟基-5-甲基异恶唑-4-丙酸酯（AMPA）受体亚基（GluR1、GluR2）mRNA表达的影响。食欲素-B 至大鼠原代皮质神经元培养物。当orexin-A和orexin-B浓度分别超过0.1μM和0.01μM时，NR1和NR2A亚基的mRNA显着减少。仅在1μM浓度的orexin-A和orexin-B也显着降低NR2B亚基的mRNA表达。此外，浓度超过0.01μM的orexin-A和orexin-B显着降低AMPA受体亚基GluR1和GluR2的mRNA表达。本研究表明，食欲素显着抑制皮质神经元培养物中 NMDA 和 AMPA 受体亚基的 RNA 表达，表明食欲素可能调节大脑皮层的高级功能，并参与下丘脑的能量调节。 (C) 2008 Elsevier Inc. 保留所有权利。
Orexin is one of the orexigenic neuropeptides in the hypothalamus. Orexin neurons in the lateral hypothalamus (LH) project into the cerebral cortex and hippocampus in which the receptors are distributed in high concentrations. Therefore, to elucidate the actions of orexin in the cerebral cortex, we examined its effects on the mRNA expressions of N-methyl-D-aspartate (NMDA) receptor subunits (NR1, NR2A, NR2B) and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunits (GluR1, GluR2) following 6-day application of orexin-A or orexin-B to rat primary cortical neuron cultures. The mRNAs of NR1 and NR2A subunits were significantly decreased by orexin-A and orexin-B at concentrations over 0.1 mu M and 0.01 mu M, respectively. The mRNA expression of NR2B subunit was also significantly decreased by orexin-A and orexin-B only at the concentration of 1 mu M. Moreover, orexin-A and orexin-B at concentrations over 0.01 mu M significantly decreased the mRNA expressions of AMPA receptor subunits, GluR1 and GluR2. The present study demonstrated that orexins significantly suppressed RNA expressions of NMDA and AMPA receptor subunits in cortical neuron cultures, suggesting that orexin may regulate the higher functions of the cerebral cortex as well as be involved in energy regulation in the hypothalamus. (C) 2008 Elsevier Inc. All rights reserved.