Purine-resistant Drosophila melanogaster result from mutations in the adenine phosphoribosyltransferase structural gene.
Purine-resistant Drosophila melanogaster result from mutations in the adenine phosphoribosyltransferase structural gene.
复制标题
嘌呤抗性黑腹果蝇是由腺嘌呤磷酸核糖转移酶结构基因突变引起的。
DOI：
10.1073/pnas.80.10.2990
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
T. Friedman
中科院分区：
文献类型：
--
作者：
D. Johnson;T. Friedman
筛选出的抗嘌呤杀死的黑腹果蝇突变体缺乏腺嘌呤磷酸核糖转移酶(APRT；E.C.2.4.2.7)活性。遗传作图和互补分析表明，嘌呤抗性、APRT活性缺失和APRT等电点的差异是由APRT(MAP位置，3：3.03)的单个基因座改变引起的。在aprt杂合子和不同aprt等位基因的单倍体果蝇中，aprt活性水平显示出基因剂量依赖性。果蝇aprt是由明显相同的23,000-道尔顿亚基组成的二聚体。这些结果表明aprt含有aprt的结构基因。
Mutants of Drosophila melanogaster selected for resistance to purine killing are deficient in adenine phosphoribosyltransferase (APRT; E.C. 2.4.2.7) activity. Genetic mapping and complementation analysis demonstrate that purine resistance, deficiency of APRT activity, and differences in the isoelectric point of APRT result from alterations at a single locus, Aprt (map position, 3:3.03). The level of APRT activity shows gene dose dependence in Aprt heterozygotes and in flies that are haploid for different Aprt alleles. Drosophila APRT is a dimer composed of apparently identical 23,000-dalton subunits. These results suggest that Aprt contains the structural gene for APRT.