Uteroglobin production in the pseudopregnant rabbit uterus. Immunohistochemical studies.
Uteroglobin production in the pseudopregnant rabbit uterus. Immunohistochemical studies.
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假孕兔子宫中子宫珠蛋白的产生。
DOI：
10.1007/bf00496819
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Gerschenson,LE
中科院分区：
文献类型：
--
作者：
Shroyer,KR;Williams,CL;Miller,GJ;Gerschenson,LE
子宫珠蛋白是家兔子宫内膜上皮的一种分泌蛋白，用直接免疫过氧化物酶法对正常和hCG诱导的假孕家兔子宫内膜上皮细胞进行了定位，在正常家兔子宫内膜上皮细胞中，子宫珠蛋白几乎局限于所有细胞的顶端细胞质中。通过hCG给药后4天，假孕激素的诱导导致参与子宫珠蛋白合成的上皮细胞的绝对数量显著持续增加。此外，内质网更强烈的子宫珠蛋白染色比对照兔子宫内膜上皮细胞。因此，对激素刺激的反应，子宫珠蛋白产量的增加似乎是通过给定细胞合成的子宫珠蛋白量的增加以及参与子宫珠蛋白合成的细胞数量的增加来实现的。与合成子宫珠蛋白的细胞数量的增加同时，未染色细胞数量的增加首先出现在假孕的第二天，在最大上皮增殖期间。然而，在注射hCG后的第二天，在那些含有子宫珠蛋白的细胞中，大多数染色仅限于核周膜。在注射hCG后的不同时间，在子宫上皮中观察到许多散在的细胞，子宫珠蛋白强烈染色。根据超微结构研究，不能排除台盼蓝，和线粒体内子宫珠蛋白的存在，它们被确定为死亡或垂死的细胞。
Uteroglobin, a secretory protein of rabbit uterine epithelium, was localized by the direct immunoperoxidase method in control and hCG-induced pseudopregnant rabbits.In control rabbits, uteroglobin was confined to the apical cytoplasm of nearly all cells of the endometrial epithelium. The induction of pseudopregnancy resulted in a pronounced continuing increase, through 4 days post-hCG administration, in the absolute number of epithelial cells engaged in uteroglobin synthesis. Furthermore, the endoplasmic reticulum was more intensely stained for uteroglobin than in the epithelial cells of control rabbit endometrium. Thus, the increased production of uteroglobin, in response to hormonal stimulation, appears to be achieved both through an increase in the amount of uteroglobin synthesized by a given cell as well as by an increase in the number of cells involved in uteroglobin synthesis.Concurrent with the increase in the number of cells synthesizing uteroglobin, an increase in the number of unstained cells first appeared at the second day of pseudopregnancy, during the period of maximal epithelial proliferation. However, within those cells containing uteroglobin on the second day following injection with hCG, most staining was limited to the perinuclear membrane.At various times following hCG administration, a number of scattered cells, intensely stained for uteroglobin, were observed in the uterine epithelium. Based upon ultrastructural studies, failure to exclude trypan blue, and the presence of intra-mitochondrial uteroglobin, they were identified as cither dead or dying cells.