Comparison of cholesteryl ester transfer from chylomicrons and other plasma lipoproteins to aortic intima media of cholesterol-fed rabbits.
Comparison of cholesteryl ester transfer from chylomicrons and other plasma lipoproteins to aortic intima media of cholesterol-fed rabbits.
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胆固醇酯从乳糜微粒和其他血浆脂蛋白转移到胆固醇喂养兔子的主动脉内膜中层的比较。
DOI：
10.1161/01.atv.2.6.493
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Zilversmit,DB
中科院分区：
文献类型：
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作者：
Stender,S;Zilversmit,DB
同时注射14c -胆固醇标记的淋巴乳糜微粒和3h -胆固醇标记的血浆极低和中密度脂蛋白（d小于1.019）。注射后1 ~ 4小时在组织中发现的乳糜微粒来源的胆固醇酯（14C）是乳糜微粒胆固醇酯直接和间接摄取的总和。乳糜微粒胆固醇酯的直接摄取被定义为在内皮细胞中乳糜微粒与脂蛋白脂肪酶相互作用时发生的原位摄取。乳糜微粒胆固醇酯的间接摄取来自于乳糜微粒残留物和其他血浆脂蛋白的摄取，这些血浆脂蛋白在注射的乳糜微粒降解过程中通过净脂质转移或交换被标记。乳糜微粒胆固醇酯对组织的间接贡献是由注射血浆中得到的组织3h -胆固醇酯d小于1.019个脂蛋白计算得出的。在连续40-60天每天喂食0.5 g胆固醇、血浆胆固醇浓度在20 - 30 mM （800-1200 mg/dl）之间的家兔中，通过直接摄取乳糜微粒胆固醇酯流入主动脉内膜的胆固醇酯不到1%，流入肝脏的胆固醇酯不到10%。
Lymph chylomicrons labeled with 14C-cholesterol and plasma very low and intermediate density lipoproteins (d less than 1.019) labeled with 3H-cholesterol were injected simultaneously into recipient cholesterol-fed rabbits. The cholesteryl ester of chylomicron origin (14C) found in the tissues 1 to 4 hours after the injection is the sum of both direct and indirect uptake of chylomicron cholesteryl ester. The direct uptake of chylomicron cholesteryl ester is defined as occurring in situ during the interaction between chylomicrons and lipoprotein lipase in endothelial cells. The indirect uptake of chylomicron cholesteryl ester results from the uptake of chylomicron remnants and of other plasma lipoproteins which become labeled during the degradation of the injected chylomicrons by net lipid transfer or by exchange. The indirect contribution of chylomicron cholesteryl ester to tissues was calculated from tissue 3H-cholesteryl ester derived from injected plasma d less than 1.019 lipoproteins. In rabbits fed 0.5 g cholesterol daily for 40-60 days, with plasma cholesterol concentrations between 20 and 30 mM (800-1200 mg/dl), less than 1% of the cholesteryl ester influx into aortic intima media and less than 10% in liver was derived from the direct uptake of chylomicron cholesteryl ester.