Lipid dependence of glucose-6-phosphate phosphohydrolase: a study with purified phospholipid transfer proteins and phosphatidylinositol-specific phospholipase C.
Lipid dependence of glucose-6-phosphate phosphohydrolase: a study with purified phospholipid transfer proteins and phosphatidylinositol-specific phospholipase C.
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DOI：
10.1021/bi00521a035
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发表时间：
1981-09
期刊：
影响因子：
2.9
通讯作者：
R. Crain;D. B. Zilversmit
中科院分区：
文献类型：
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作者：
R. Crain;D. B. Zilversmit
Richard C. Crain 1和Donald B。Zilversmit*· 5摘要：利用牛肝非特异性和磷脂酰胆碱特异性转运蛋白以及金黄色葡萄球菌磷脂酰肌醇特异性磷脂酶C修饰微粒体膜磷脂组成，研究葡萄糖-6-磷酸水解酶（EC 3.1. 3.9)对膜磷脂的活性孵育的微粒体与二棕榈酰磷脂酰胆碱含有单层囊泡和任一转移蛋白产生的膜中，这disaturated磷脂贡献了43%的总磷脂酰胆碱。孵育的微粒体和α-磷脂酰胆碱单层囊泡与非特异性转移蛋白引起的膜磷脂酰胆碱含量的净增加和磷脂酰乙醇胺和磷脂酰肌醇水平的净减少，而在孵育的磷脂酰胆碱转移蛋白，磷脂类组合物没有发生变化。将微粒体和磷脂酰胆碱/磷脂酰乙醇胺（3：1 mol/mol）单层囊泡与非特异性转移蛋白孵育，也会导致磷脂酰胆碱含量增加和磷脂酰肌醇含量减少。已发现，膜结合酶的数量对脂质双层具有功能依赖性（Sander-mann，1978）。Fleischer及其同事（1962）清楚地证明了这一点，他们首先通过（a）去除磷脂，
Richard C. Crain1 and Donald B. Zilversmit*· 5 abstract: The nonspecific and phosphatidylcholine-specific transfer proteins from beef liver and the phosphatidylinositol-specific phospholipase C from Staphylococcus aureus were used to modify the phospholipid composition of micro-somal membranes in order to study the dependence of glu-cose-6-phosphate phosphohydrolase (EC 3.1. 3.9) activity on membrane phospholipids. Incubation of microsomes with dipalmitoylphosphatidylcholine-containing unilamellar vesicles and either transfer protein produced a membrane in which this disaturated phospholipid contributed up to 43% of the total phosphatidylcholine. Incubation of microsomes and phos-phatidylcholine unilamellar vesicles with nonspecific transfer protein caused a net increase in the membrane phosphatidylcholine content and a net decrease in the phosphatidylethanolamine and phosphatidylinositol levels, whereas in incubations with the phosphatidylcholine transfer protein, no change in phospholipid class composition occurred. Incubations of microsomes and phosphatidylcholine/phosphatidylethanolamine (3: 1 mol/mol) unilamellar vesicles with the nonspecific transfer protein alsocaused an increased phos-phatidylcholine content and a decreased phosphatidylinositolINíumerous membrane-bound enzymes have been found to have a functional dependence on the lipid bilayer (Sander-mann, 1978). This was clearly demonstrated by Fleischer and co-workers (1962) who first showed a role of phospholipids in the mitochondrial electron transfer system by (a) removing