Cellular and matrix mechanics of bioartificial tissues during continuous cyclic stretch.
Cellular and matrix mechanics of bioartificial tissues during continuous cyclic stretch.
复制标题
连续循环拉伸期间生物人工组织的细胞和基质力学。
DOI：
10.1007/s10439-006-9153-1
复制
发表时间：
2006
影响因子：
3.8
通讯作者：
Okamoto,RuthJ
中科院分区：
文献类型：
--
作者：
Wille,JeremiahJ;Elson,ElliotL;Okamoto,RuthJ
Bioartificial tissues are useful model systems for studying cell and extra-cellular matrix mechanics. These tissues provide a 3D environment for cells and allow tissue components to be easily modified and quantified. In this study, we fabricated bioartificial tissue rings from a 1 ml solution containing one million cardiac fibroblasts and 1 mg collagen. After 8 days, rings compacted to <1% of original volume and cell number increased 2.4 fold. We initiated continuous cyclic stretching of the rings after 2, 4, or 8 days of incubation, while monitoring the tissue forces. Peak tissue force during each cycle decreased rapidly after initiating stretch, followed by further slow decline. We added 2 μM Cytochalasin-D to some rings prior to initiation of stretch to determine the force contributed by the matrix. Cell force was estimated by subtracting matrix force from tissue force. After 12 h, matrix force-strain curves were highly nonlinear. Cell force-strain curves were linear during loading and showed hysteresis indicating viscoelastic behavior. Cell stiffness increased with stretching frequency from 0.001–0.25 Hz. Cell stiffness decreased with stretch amplitude (5–25%) at 0.1 Hz. The trends in cell stiffness do not fit simple viscoelastic models previously proposed, and suggest possible strain-amplitude related changes during cyclic stretch.
