Rapid diagnostic method for discriminating two types of COI sequences in the gall-forming aphid Tetraneura nigriabdominalis(Hemiptera : Aphididae) by multiplex polymerase chain reaction
Rapid diagnostic method for discriminating two types of COI sequences in the gall-forming aphid Tetraneura nigriabdominalis(Hemiptera : Aphididae) by multiplex polymerase chain reaction
复制标题
多重聚合酶链式反应快速鉴别黑腹蚜虫（半翅目：蚜科）两类 COI 序列的方法
DOI：
10.1111/j.1479-8298.2012.00557.x
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发表时间：
2013
影响因子：
0.9
通讯作者：
S
中科院分区：
文献类型：
--
作者：
Lee W;Otsuki A;Akimoto;S
黑腹叶蝉Tetraneura nigriabitalis(Sasaki，1899)是一种广泛分布于欧亚大陆的害虫。在本研究中，建立了一种诊断多重聚合酶链式反应的方法来区分该物种的两种细胞色素氧化酶亚基 I(COI)序列，并从25份黑腹 样本中检验了该方法的有效性。多重聚合酶链式反应和凝胶电泳法显示，10份样品和15份样品分别有1条133bP( A型)和426pB型( B型)条带。这种区分与根据COI序列(511 碱基)对25个样本的划分是一致的，表明该方法在区分这两种类型方面具有很高的准确性。该方法可用于研究这两种类型的微地理分布和黑腹 的遗传多样性。
Tetraneura nigriabdominalis(Sasaki, 1899) is a galling aphid that is distributed widely in Eurasia. In the present study, a diagnostic multiplex polymerase chain reaction (PCR) protocol was developed to discriminate two types of the cytochrome oxidase subunit I (COI) sequences in this species, and its usefulness was examined from 25 samples ofT. nigriabdominalis. Multiplex PCR and electrophoresis revealed that 10 and 15 samples had one band of 133 bp (type A) and 426 bp (type B), respectively. This discrimination was identical to the division of the 25 samples based on the alignments ofCOIsequences (511 bp), indicating high accuracy of this protocol in distinguishing the two types. This protocol is useful for studying microgeographic distributions of the two types and genetic diversities inT. nigriabdominalis.