Intraduodenal Infusion of Lysophosphatidylcholine Restores the Intestinal Absorption of Vitamins a and E in Rats Fed a Low-Zinc Diet
Intraduodenal Infusion of Lysophosphatidylcholine Restores the Intestinal Absorption of Vitamins a and E in Rats Fed a Low-Zinc Diet
复制标题
十二指肠内输注溶血磷脂酰胆碱可恢复低锌饮食大鼠肠道对维生素 A 和 E 的吸收
DOI：
--
复制
发表时间：
2001
影响因子：
3.2
通讯作者：
S. Koo
中科院分区：
文献类型：
--
作者：
S. Noh;S. Koo
我们之前的工作表明，在喂食低锌（LZ）饮食的大鼠中，脂质和脂溶性维生素、视黄醇和α-生育酚（αTP）的淋巴吸收显着降低，同时淋巴磷脂输出也降低。磷脂酰胆碱 (PC) 肠内输注后，可恢复脂肪和视黄醇的吸收，但进一步降低喂食 LZ 饮食的大鼠对 αTP 的吸收。本研究旨在确定腔内输注溶血磷脂酰胆碱（胰磷脂酶 A2 (PLA2) 水解 PC 的产物）是否会同时恢复 LZ 大鼠对视黄醇和 αTP 的吸收。老鼠被训练每天吃两顿饭，并被分成两组。一组喂食含有 LZ（3.0 mg Zn/kg）的 AIN-93G 饮食，另一组喂食相同的饮食，但含有足够的锌（AZ；30.0 mg Zn/kg），持续 6 周。将含有视黄醇、αTP 和 14C 标记的三油酸甘油酯（14C-油酸）、含或不含 1-油酰基-2-羟基磷脂酰胆碱 (lysoPC) 的脂肪乳剂以 3.0 ml/hr 的速度输注在 24 ml PBS (pH 6.5) 中，持续 8 小时。当输注不含lysoPC的脂肪乳剂时，LZ大鼠中视黄醇和αTP的吸收（视黄醇，13.2±1.5nmol；αTP，430.6±66.8nmol）显着低于AZ大鼠（视黄醇，18.2±1.0nmol；αTP，543.8±58.9nmol）。 LZ 大鼠中维生素吸收的降低与 14C-油酸吸收的显着降低同时发生。然而，当输注含有 lysoPC 的乳液时，LZ 大鼠中维生素的吸收（视黄醇，18.4 ± 3.0 nmol；αTP，777.2 ± 92.1 nmol）完全恢复至对照水平（视黄醇，20.4 ± 2.8 nmol；αTP，756.3 ± 136.1 nmol）。结果表明，LZ 大鼠中 PLA2 将 PC 腔内水解为溶血 PC 的过程可能受到损害，导致脂肪和脂溶性维生素的吸收受损。
Our previous work has shown that the lymphatic absorptions of lipids and lipid-soluble vitamins, retinol and α-tocopherol (αTP), are lowered markedly in rats fed a low-zinc (LZ) diet in parallel with lower lymphatic phospholipid outputs. Phosphatidylcholine (PC), when infused enterally, restored the absorptions of fat and retinol, but further lowered the absorption of αTP in rats fed the LZ diet. This study was conducted to determine whether a luminal infusion of lysophosphatidylcholine, a product of PC hydrolysis by pancreatic phospholipase A2 (PLA2), would simultaneously restore the absorptions of retinol and αTP in LZ rats. Rats were trained to consume two meals per day and were divided into two groups. One group was fed an AIN-93G diet containing a LZ (3.0 mg Zn/kg), and the other was fed the same diet, but containing adequate zinc (AZ; 30.0 mg Zn/kg) for 6 weeks. Rats with lymph cannula were infused at 3.0 ml/hr for 8 hr with a lipid emulsion containing retinol, αTP, and 14C-labeled triolein (14C-oleic acid) with or without 1-oleoyl-2-hydroxy phosphatidyicholine (lysoPC) in 24 ml of PBS (pH 6.5). When the lipid emulsion without lysoPC was infused, the absorptions of retinol and αTP were significantly lower in LZ rats (retinol, 13.2 ± 1.5 nmol; αTP, 430.6 ± 66.8 nmol) than in AZ rats (retinol, 18.2 ± 1.0 nmol; αTP, 543.8 ± 58.9 nmol). The lower absorptions of the vitamins in LZ rats occurred in parallel with a significant decrease in 14C-oleic acid absorption. When the emulsion containing lysoPC was infused, however, absorptions of the vitamins (retinol, 18.4 ± 3.0 nmol; αTP, 777.2 ± 92.1 nmol) in LZ rats were restored completely to the control levels (retinol, 20.4 ± 2.8 nmol; αTP, 756.3 ± 136.1 nmol). The results suggest that the luminal hydrolysis of PC to lysoPC by PLA2 may be impaired in LZ rats, resulting in impaired absorption of fat and the fat-soluble vitamins.
通过高效液相色谱法分离和定量视黄酯和视黄醇。
影响因子：
--
作者：
Ross,AC
通讯作者：
Ross,AC