Interaction of environmental Burkholderia cenocepacia strains with cystic fibrosis and non-cystic fibrosis bronchial epithelial cells in vitro
Interaction of environmental Burkholderia cenocepacia strains with cystic fibrosis and non-cystic fibrosis bronchial epithelial cells in vitro
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DOI：
10.1099/mic.0.056986-0
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发表时间：
2012-05-01
期刊：
影响因子：
2.8
通讯作者：
McClean, Siobhan
中科院分区：
文献类型：
--
作者：
Bevivino, Annamaria;Pirone, Luisa;McClean, Siobhan
新洋葱伯克霍尔德菌是囊性纤维化（CF）患者的重要病原体。非临床宿主可能在感染的获得中起作用，因此评估环境B的致病潜力是重要的。新洋葱分离物。在这项研究中，我们调查了两个环境B的相互作用。新洋葱球菌菌株（Mex 1和MCII-168）与两种支气管上皮细胞系16 HBE 14 o（-）和CFBE 41 o（-）（其分别具有非CF和CF表型）。与临床B相比，环境菌株对CF和非CF细胞的侵袭水平显著降低。新洋葱LMG 16656（T）菌株。极化CFBE 41 o（-）或16 HBE 14 o（-）细胞暴露于环境菌株导致跨上皮电阻（TER）显著降低，与暴露于临床菌株后观察到的结果相当。在16 HBE 41 o（-）细胞中，环境菌株导致TER下降，而紧密连接蛋白如紧密连接蛋白ZO-1没有明显影响。相比之下，在CF细胞中，ZO-1的量及其定位明显改变了两种环境菌株的存在，与LMG 16656的效果相当。这项研究表明，即使环境菌株的侵袭性明显低于临床菌株，它们对上皮完整性的影响也与临床菌株相当。最后，与表达功能性囊性纤维化跨膜调节因子（CFTR）的细胞相比，紧密连接调节蛋白ZO-1似乎更容易受到CF细胞中环境菌株存在的影响。
Burkholderia cenocepacia is an important human pathogen in patients with cystic fibrosis (CF). Non-clinical reservoirs may play a role in the acquisition of infection, so it is important to evaluate the pathogenic potential of environmental B. cenocepacia isolates. In this study, we investigated the interactions of two environmental B. cenocepacia strains (Mex1 and MCII-168) with two bronchial epithelial cell lines, 16HBE14o(-) and CFBE41o(-), which have a non-CF and a CF phenotype, respectively. The environmental strains showed a significantly lower level of invasion into both CF and non-CF cells in comparison with the clinical B. cenocepacia LMG16656(T) strain. Exposure of polarized CFBE41o(-) or 16HBE14o(-) cells to the environmental strains resulted in a significant reduction in transepithelial resistance (TER), comparable with that observed following exposure to the clinical strain. A different mechanism of tight junction disruption in CF versus non-CF epithelia was found. In the 16HBE41o(-) cells, the environmental strains resulted in a drop in TER without any apparent effect on tight junction proteins such as zonula occludens-1 (ZO-1). In contrast, in CF cells, the amount of ZO-1 and its localization were clearly altered by the presence of both the environmental strains, comparable with the effect of LMG16656. This study demonstrates that even if the environmental strains are significantly less invasive than the clinical strain, they have an effect on epithelial integrity comparable with that of the clinical strain. Finally, the tight junction regulatory protein ZO-1 appears to be more susceptible to the presence of environmental strains in CF cells than in cells which express a functional cystic fibrosis transmembrane regulator (CFTR).