3D mesoscopic architecture of a heterogeneous cellular network in the cementum?periodontal ligament?alveolar bone complex
3D mesoscopic architecture of a heterogeneous cellular network in the cementum?periodontal ligament?alveolar bone complex
复制标题
牙骨质、牙周膜、牙槽骨复合体中异质细胞网络的 3D 细观结构
DOI：
10.1093/jmicro/dfab051
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发表时间：
2021
期刊：
影响因子：
1.8
通讯作者：
Nakamura Kei-ichiro
中科院分区：
文献类型：
--
作者：
Hirashima Shingo;Ohta Keisuke;Togo Akinobu;Nakamura Kei-ichiro
细胞间的通讯协调了各种细胞和组织的功能。这种交流使细胞通过细胞间连接的直接接触形成细胞网络。由于这些细胞网络与组织和器官功能密切相关，阐明细胞网络的形态特征可能导致新的治疗方法的发展。牙齿、牙周韧带（PDL）和牙槽骨通过胶原纤维形成复合物。牙齿依靠这个复合体的协调活动来维持其功能，它的三个组成部分都有细胞网络。三维（3D）介观结构分析的成像方法包括聚焦离子束/扫描电子显微镜（FIB/SEM），其特点是能够在广泛的块面成像后选择观察点并从复杂组织中获取数据，而无需制备大量超薄切片。之前，我们使用FIB/SEM分析了硬组织的三维介观结构，包括存在于骨和牙根之间的PDL。影像学结果显示牙骨质、牙槽骨和牙槽骨网络相互接触，形成异质细胞网络。这种细胞网络可以协调机械负荷诱导的骨质-牙槽骨-牙髓骨复合体的重建，因为每个复合体的重建是协调的，例如正畸治疗导致的牙齿移动和咬合丧失导致的牙齿脱位。在这篇综述中，我们总结和讨论了在我们最近的介观和形态学研究中观察到的骨质，PDL和牙槽骨中细胞网络的三维介观结构。
Cell-to-cell communication orchestrates various cell and tissue functions. This communication enables cells to form cellular networks with each other through direct contact via intercellular junctions. Because these cellular networks are closely related to tissue and organ functions, elucidating the morphological characteristics of cellular networks could lead to the development of novel therapeutic approaches. The tooth, periodontal ligament (PDL) and alveolar bone form a complex via collagen fibres. Teeth depend on the co-ordinated activity of this complex to maintain their function, with cellular networks in each of its three components. Imaging methods for three-dimensional (3D) mesoscopic architectural analysis include focused ion beam/scanning electron microscopy (FIB/SEM), which is characterized by its ability to select observation points and acquire data from complex tissue after extensive block-face imaging, without the need to prepare numerous ultrathin sections. Previously, we employed FIB/SEM to analyse the 3D mesoscopic architecture of hard tissue including the PDL, which exists between the bone and tooth root. The imaging results showed that the cementum, PDL and alveolar bone networks are in contact and form a heterogeneous cellular network. This cellular network may orchestrate mechanical loading–induced remodelling of the cementum–PDL–alveolar bone complex as the remodelling of each complex component is coordinated, as exemplified by tooth movement due to orthodontic treatment and tooth dislocation due to occlusal loss. In this review, we summarize and discuss the 3D mesoscopic architecture of cellular networks in the cementum, PDL and alveolar bone as observed in our recent mesoscopic and morphological studies.