Decreased endothelial cell survival after transplantation of corneas preserved by three modifications of corneal organ culture technique.
Decreased endothelial cell survival after transplantation of corneas preserved by three modifications of corneal organ culture technique.
复制标题
通过角膜器官培养技术的三种改进保存的角膜移植后内皮细胞存活率降低。
DOI：
10.1016/s0161-6420(85)33834-4
复制
发表时间：
1985
期刊：
影响因子：
13.7
通讯作者：
Lindstrom,RL
中科院分区：
文献类型：
--
作者：
Bourne,WM;Doughman,DJ;Lindstrom,RL
将人供体角膜置于4°C的M-K培养液中培养24小时，在34℃的最低基本培养液中培养2~5周，然后(1)将角膜在4℃的M-K培养液中培养48小时(组1，47只眼)，(2)在移植前将角膜置于4°C的M-K培养液中(组2，17只眼)，或(3)将角膜移植到不经培养后冷却至4℃的角膜(组3，11只眼)。我们将器官培养保存的角膜与同期移植的相同数量的角膜进行了比较，但只在4℃的M-K培养液中保存了一到四天。在三组中的每一组中，器官培养的角膜移植后两个月观察到的中央内皮细胞丢失明显大于M-K保存的角膜移植。3组11只器官培养角膜的平均内皮细胞损失率明显低于1、2组器官培养的角膜。1组角膜移植后1年复查，器官培养的角膜内皮细胞损失量仍明显大于M-K保存的移植角膜。这些结果表明，供体角膜在34℃的最低基本培养液中培养数周(在4℃的M-K培养液中保存24小时后)会降低内皮细胞在角膜移植后存活的能力，而在4℃的M-K培养液中培养后的培养进一步降低了这种能力。中心内皮细胞存活率的摄影检测比临床成功率更敏感，在器官培养和M-K保存的移植物中，两者的成功率相同(96%)。角膜保存的新方法需要用内皮细胞检测技术来检验。
We placed human donor corneas in M-K medium at 4° C for 24 hours, cultured them in minimal essential medium at 34° C for two to five weeks, and then either (1) placed the corneas in M-K medium at 4° C for 48 hours before transplantation (Group 1, 47 eyes); (2) placed the corneas in M-K medium at 4° C for 16 hours before transplantation (Group 2,17 eyes); or (3) transplanted the corneas without postculture cooling to 4° C (Group 3, 11 eyes). We compared the corneas preserved by organ culture with an equal number of corneas transplanted during the same period, but preserved only in M-K medium at 4° C for one to four days. The central endothelial cell losses noted two months after keratoplasty were significantly greater in the organ-cultured corneas than in the M-K-preserved corneas in each of the three groups. The mean endothelial cell loss in the 11 organ-cultured corneas in Group 3 was significantly less than that in the 64 organ-cultured corneas in Groups 1 and 2. The corneas in Group 1 were also examined one year after keratoplasty, and the cell losses in the organ-cultured grafts remained significantly greater than those in the M-K-preserved grafts. These results indicate that the culture of donor corneas in minimal essential medium at 34° C for several weeks (after storage in M-K medium at 4° C for 24 hours) decreases the ability of the endothelial cells to survive the trauma of keratoplasty, and that postculture storage at 4° C in M-K medium decreases this ability further. Photographic assay of central endothelial cell survival was a more sensitive indicator of the efficacy of the method of corneal preservation than was the clinical success rate, which was the same (96%) in both the organ-cultured and M-K-preserved grafts. New methods of corneal preservation should be tested by the endothelial cell assay technique.
登录
查看更多内容
减少有晶状体眼穿透性角膜移植术期间内皮细胞的损失。
DOI：
--
发表时间：
1980
期刊：
American journal of ophthalmology-glaucoma
影响因子：
--
作者：
William M. Bourne
通讯作者：
William M. Bourne
器官培养中长期保存供体角膜：长期临床评估。
DOI：
--
发表时间：
1980
期刊：
影响因子：
--
作者：
Doughman Dj
通讯作者：
Doughman Dj
使用双线缝合技术进行穿透性角膜移植术的结果。
DOI：
10.1001/archopht.1981.03930020465012
发表时间：
1981
期刊：
Archives of ophthalmology (Chicago, Ill. : 1960)
影响因子：
--
作者：
Davison,JA;Bourne,WM
通讯作者：
Bourne,WM
穿透性角膜移植术期间内皮细胞损失。
DOI：
--
发表时间：
1978
期刊：
American journal of ophthalmology-glaucoma
影响因子：
--
作者：
William M. Bourne;W. O'Fallon
通讯作者：
W. O'Fallon