Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat
Root architecture and leaf photosynthesis traits and associations with nitrogen-use efficiency in landrace-derived lines in wheat
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DOI：
10.1016/j.eja.2022.126603
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发表时间：
2022-08-26
影响因子：
5.2
通讯作者：
Foulkes, M. John
中科院分区：
文献类型：
--
作者：
Kareem, Shadia H. S.;Hawkesford, Malcolm J.;Foulkes, M. John
根系构型在优化氮素利用方面具有重要意义。高通量表型分析技术可用于在受控环境下研究根系构型性状。利用由发芽纸基袋和灯芯结合图像分析组成的根系表型分析平台，在水培高氮（HN）和低氮（LN）条件下，对30个来源于地方品种的面包小麦基因型和面包小麦亲本Paragon的根系幼苗性状进行了分析。此外，还对13个小麦基因型（其中8个基因型与根系表型相同）在高氮和低氮条件下进行了两个温室试验，测定了旗叶光合速率、氮吸收量和单株生物量等全株表现。在HN和LN条件下，单株种子根数、单株侧根数、单株种子根长和种子根角度等RSA性状在基因型间存在显著差异，地方品种的RSA性状在亲本Paragon之上存在超亲分离。在温室条件下，旗叶光合速率的遗传变异范围为25.9-33.3 μ mol m ~（-2）s ~（-1），吸氮量的遗传变异范围为0.37-0.48 gN株~（-1）和0.21-0.30 gN株~（-1）（P < 0.05），均为Paragon以上的超亲分离。在HN和LN条件下，氮素营养指数在Paragon以上的地方品种中也表现出超亲分离。更大的最大根深和更多的侧根，每株植物在水培屏幕分别与LN条件下增加的生物量。这项研究的结果表明，以上的精英亲本品种Paragon，这可能是用来提高育种计划中的N-利用效率的地方品种衍生线的幼苗RSA性状的遗传变异。
Root system architecture (RSA) is important in optimizing the use of nitrogen. High-throughput phenotyping techniques may be used to study root system architecture traits under controlled environments. A root pheno-typing platform, consisting of germination paper-based pouch and wick coupled with image analysis, was used to characterize root seedling traits in 30 landrace-derived bread wheat genotypes and the bread wheat parent Paragon under hydroponic high N (HN) and low N (LN) conditions. In addition, two glasshouse experiments under HN and LN conditions were carried out to measure whole plant performance including flag-leaf photo-synthetic rate, N uptake and biomass per plant for 13 wheat genotypes of which eight were common with those in the root phenotyping hydroponic experiment. There were significant differences in RSA traits between ge-notypes for seminal root number per plant, lateral root number per plant, seminal root length per plant and seminal root angle, with transgressive segregation for landrace-derived lines above the elite parental cultivar Paragon under HN and LN conditions. In the glasshouse experiments, genetic variation in flag-leaf photosynthesis rate was found in landrace-derived genotypes in the range 25.9-33.3 mu mol m- 2 s- 1 under HN and in N uptake in the ranges 0.37-0.48 g N plant-1and 0.21-0.30 g plant-1 under HN and LN conditions, respectively (P < 0.05), with transgressive segregation above Paragon. Plant Nitrogen Nutrition Index also showed transgressive segre-gation in the landrace-derived lines above Paragon under HN and LN conditions. Greater maximum root depth and more lateral roots per plant in the hydroponic screen were each correlated with increased biomass per plant under LN conditions. Results from this study demonstrated genetic variation for seedling RSA traits in landrace-derived lines above the elite parental cultivar Paragon, which potentially could be utilized to improve N-use efficiency in breeding programmes.