Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways
Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways
复制标题
牛蒡甙元部分通过调节抗氧化途径来增强久坐大鼠的游泳耐力。
DOI：
10.1038/aps.2014.70
复制
发表时间：
2014-10-01
影响因子：
8.2
通讯作者：
Shen, Xu
中科院分区：
文献类型：
--
作者：
Wu, Ruo-ming;Sun, Yan-yan;Shen, Xu
目的：牛蒡子苷元（Arctigenin）是一种存在于中草药中的苯丙素类二苄基丁内酯木脂素，具有抗肿瘤、抗炎、神经保护和增强耐力等多种药理活性。方法：采用H_2O_2（700 μ mol/L）染毒大鼠L_6骨骼肌细胞，以DCFH-DA为探针，检测细胞内活性氧（ROS）水平。雄性SD大鼠腹腔注射牛蒡子苷元15 d，连续6周，然后进行负重强迫游泳试验（WFST），评价大鼠的耐力。mg.kg结果：牛蒡子苷元（1、5和20 μ mol/L）可剂量依赖性地抑制H2 O2诱导的L 6细胞和大鼠骨骼肌中ROS的产生。WFST结果表明长期给予牛蒡子苷元显著提高大鼠的耐力。此外，对大鼠L 6细胞和骨骼肌的分子生物学研究表明，牛蒡子苷元通过调节两种潜在的抗氧化途径，有效地增加抗氧化剂相关基因的表达，包括超氧化物歧化酶（SOD）、谷胱甘肽还原酶（Gsr）、谷胱甘肽过氧化物酶（GPX 1）、硫氧还蛋白（Txn）和解偶联蛋白2（UCP 2）：结论：牛蒡子苷元通过提高骨骼肌的抗氧化能力，有效地提高大鼠游泳耐力，从而突出了这种天然产物作为抗氧化剂在治疗疲劳和相关疾病中的潜力。
Aim: Arctigenin, a phenylpropanoid dibenzylbutyrolactone lignan found in traditional Chinese herbs, has been determined to exhibit a variety of pharmacological activities, including anti-tumor, anti-inflammation, neuroprotection, and endurance enhancement. In the present study, we investigated the antioxidation and anti-fatigue effects of arctigenin in rats.Methods: Rat L6 skeletal muscle cell line was exposed to H2O2 (700 mu mol/L), and ROS level was assayed using DCFH-DA as a probe. Male SD rats were injected with arctigenin (15 mg.kg(-1).d(-1),ip) for 6 weeks, and then the weight-loaded forced swimming test (WFST) was performed to evaluate their endurance. The levels of antioxidant-related genes in L6 cells and the skeletal muscles of rats were analyzed using real-time RT-PCR and Western blotting.Results: Incubation of L6 cells with arctigenin (1, 5, and 20 mu mol/L) dose-dependently decreased the H2O2-induced ROS production. WFST results demonstrated that chronic administration of arctigenin significantly enhanced the endurance of rats. Furthermore, molecular biology studies on L6 cells and skeletal muscles of the rats showed that arctigenin effectively increased the expression of the antioxidant-related genes, including superoxide dismutase (SOD), glutathione reductase (Gsr), glutathione peroxidase (GPX1), thioredoxin (Txn) and uncoupling protein 2 (UCP2), through regulation of two potential antioxidant pathways: AMPK/PGC-1 alpha/PPAR alpha in mitochondria and AMPK/p53/Nrf2 in the cell nucleus.Conclusion: Arctigenin efficiently enhances rat swimming endurance by elevation of the antioxidant capacity of the skeletal muscles, which has thereby highlighted the potential of this natural product as an antioxidant in the treatment of fatigue and related diseases.