Optical biosensor assay (OBA).
Optical biosensor assay (OBA).
复制标题
光学生物传感器测定（OBA）。
作者：
Y. Tsay;C. I. Lin;J. Lee;E. Gustafson;R. Appelqvist;P. Magginetti;R. Norton;N. Teng;D. Charlton
我们描述了一种涉及光学衍射的新型生物传感器免疫测定法，用于检测人体体液中临床上重要的分析物。硅片用作固定抗原或抗体的载体。通过光掩模照射蛋白质包被的表面，以产生活性和非活性蛋白质的不同周期性区域。当表面与阳性样品一起孵育时，抗原-抗体结合仅发生在活性区域。当用诸如激光的光源照射时，所产生的生物衍射光栅使光发生衍射。阴性样品不会产生衍射，因为没有发生抗原抗体结合来产生衍射光栅。衍射信号的存在或不存在可区分阳性样品和阴性样品，并且信号的强度提供了分析物浓度的定量测量。该技术通过血清中绒毛膜促性腺激素的定量测定得到证明。
We describe a new biosensor immunoassay involving optical diffraction to detect clinically important analytes in human body fluids. A silicon wafer is used as a support for immobilization of antigen or antibody. The protein-coated surface is illuminated through a photo mask to create distinct periodic areas of active and inactive protein. When the surface is incubated with a positive sample, antigen-antibody binding occurs only on the active areas. Upon illumination with a light source such as a laser, the resulting biological diffraction grating diffracts the light. A negative sample does not result in diffraction because no antigen-antibody binding occurs to create the diffraction grating. The presence or absence of a diffraction signal differentiates between positive and negative samples, and the intensity of the signal provides a quantitative measure of the analyte concentration. The technique is demonstrated with a quantitative assay of choriogonadotropin in serum.