RECIPROCAL EXPRESSION OF LAMININ A-CHAIN ISOFORMS ALONG THE CRYPT-VILLUS AXIS IN THE HUMAN SMALL-INTESTINE
RECIPROCAL EXPRESSION OF LAMININ A-CHAIN ISOFORMS ALONG THE CRYPT-VILLUS AXIS IN THE HUMAN SMALL-INTESTINE
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DOI：
10.1016/0016-5085(94)90740-4
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发表时间：
1994-04-01
期刊：
影响因子：
29.4
通讯作者：
VACHON, PH
中科院分区：
文献类型：
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作者：
BEAULIEU, JF;VACHON, PH
背景/目标：肠上皮细胞增殖、迁移和分化易受沿着隐窝-绒毛轴的各种影响，包括基底膜的组成变化和这些组分的受体的差异表达。然而，层粘连蛋白一直检测到上皮基底膜没有显着的变化有关的隐窝绒毛功能单位。本研究的目的是检查单个层粘连蛋白链的分布。研究方法：层粘连蛋白A、M（A链变体）、B1和B2链以及整合素α 6 β 1和α 6 β 4（两种层粘连蛋白受体）的分布模式通过间接免疫荧光法在成人小肠中测定。结果：层粘连蛋白链沿隐窝-绒毛轴沿着有明显不同的表达模式。发现A链主要与绒毛上的分化上皮细胞相关，而M链仅限于隐窝的基底膜。然而，B1和B2链都被检测到沿肠基底膜沿着。此外，发现α 6 β 1和α 6 β 4整合素由所有肠上皮细胞表达。结论：这些观察结果表明，层粘连蛋白亚型的相互表达沿着隐窝绒毛轴和支持的概念，肠上皮基底膜受到复杂的成分变化。
Background/Aims: Intestinal epithelial cell proliferation, migration, and differentiation are susceptible to various influences along the crypt-villus axis including compositional changes in the basement membrane and differential expression of receptors for these components. However, laminin has been consistently detected at the epithelial basement membrane without significant variation in relation to the crypt-villus functional unit. The aim of this study was to examine the distribution of individual laminin chains. Methods: The patterns of distribution of laminin A, M (an A-chain variant), B1, and B2 chains as well as the integrins alpha6beta1 and alpha6beta4, two laminin receptors, were determined by indirect immunofluorescence in the adult human small intestine. Results: Markedly distinct patterns of laminin chain expression were observed along the crypt-villus axis. The A chain was found predominantly associated with the differentiated epithelial cells on the villus whereas the M chain was restricted to the basement membrane of crypts. However, both B1 and B2 chains were detected all along the intestinal basement membrane. Furthermore, alpha6beta1 and alpha6beta4 integrins were found to be expressed by all enterocytes. Conclusions: These observations show a reciprocal expression of laminin isoforms along the crypt-villus axis and support the concept that the intestinal epithelial basement membrane is subjected to intricate compositional changes.