Quantification of Drugs in Brain Samples.
Quantification of Drugs in Brain Samples.
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脑样本中药物的定量。
DOI：
10.1093/jat/bkv078
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发表时间：
2015
影响因子：
2.5
通讯作者：
T. Stimpfl
中科院分区：
文献类型：
--
作者：
B. Reiter;T. Stimpfl
由于缺乏经认证的参考材料，在复杂的死后基质中的可靠定量只能通过添加同位素标记的标准品或通过标准添加法来进行。虽然标准加入是基本的定量程序，但其实际实施具有挑战性，并且高度可重复的提取方法至关重要。因此，使用9种化合物强化的猪脑代表真实的尸检环境，针对尸检分析的特定要求验证了自动固相萃取筛选程序。选择验证参数线性、重复性、重现性和回收率，以检测方法的“适用性”。所有获得的结果是令人满意的，虽然该方法没有优化所选的化合物。因此，该经验证的方法可用于进一步的标准加入实验，以评价初始估计浓度对计算浓度以建立校准曲线的重要性。我们可以表明，当估计的浓度接近真实的浓度时，获得最佳结果。在偏离的情况下，低估比高估更有利。
Due to the lack of certified reference material, a reliable quantification in complex postmortem matrices can only be performed either by the addition of isotope-labelled standards or by the standard addition method. Although standard addition is a basic quantification procedure, its practical implementation is challenging, and a highly reproducible extraction method is essential. Therefore, an automated solid-phase extraction screening procedure was validated in respect to the specific requirements in postmortem analyses, using pig brain fortified with nine compounds to represent a realistic postmortem setting. The validation parameters linearity, repeatability, reproducibility and recovery were selected to test the method's 'fitness for purpose'. All obtained results were satisfying, although the method was not optimized for the selected compounds. Therefore, this validated method could be used for further standard addition experiments to evaluate the importance of the initial estimated concentration for the calculation of concentrations to set up the calibration curve. We could show that the best results were obtained when the estimated concentration was close to the real concentration. In case of a deviation, an underestimation is more favorable than an overestimation.