Gut mycobiome and its interaction with diet, gut bacteria and alzheimer's disease markers in subjects with mild cognitive impairment: A pilot study
Gut mycobiome and its interaction with diet, gut bacteria and alzheimer's disease markers in subjects with mild cognitive impairment: A pilot study
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DOI：
10.1016/j.ebiom.2020.102950
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发表时间：
2020-09-01
期刊：
影响因子：
11.1
通讯作者：
Yadav, Hariom
中科院分区：
文献类型：
--
作者：
Nagpal, Ravinder;Neth, Bryan J.;Yadav, Hariom
背景资料：最近，我们报告说，轻度认知障碍（MCI）患者在他们的肠道中携带特定的细菌签名，并且改良的地中海生酮饮食（MMKD）改善了脑脊液（CSF）中的阿尔茨海默病（AD）标志物和肠道细菌的签名。然而，其他微生物种群，例如肠道真菌方法：我们通过对17名老年人的真菌rRNA ITS 1基因进行测序，MCI 11例; 6认知正常[CN]），在一项单中心、随机、双盲、交叉初步研究中，在MMKD和美国心脏协会饮食（AHAD）干预6周前后，并确定其与CSF中AD标志物和肠道细菌的相关性。与CN患者相比，MCI患者具有较高比例的核盘菌科、棕囊菌科、毛孢菌科、囊丝菌科、披毛菌科和葡萄孢属，Kazachstania、Phaeoacremonium和Cladosporium，Meyerozyma丰度较低。特定的真菌类群表现出不同的相关性阵列与AD标志物和肠道细菌的受试者与MCI。MMKD在MCI受试者中诱导对真菌多样性的更广泛的影响，并增加蘑菇和Mrakia，同时减少酵母菌和麦角菌，在有或没有MCI的受试者中具有差异反应。解释：该研究揭示了MCI特异性真菌生物群特征，并表明不同的饮食调节MCI患者中与AD标志物和真菌-细菌共调节网络相关的真菌生物群。这些发现证实了将肠道菌群视为一个独特因素的概念，该因素可以通过与肠道细菌和饮食相互作用来影响认知健康/AD，并使用独特的饮食或微生物组调节剂促进对AD和相关微生物组的更好理解。(C)2020年，任作家。由爱思唯尔公司出版
Background: Recently, we reported that patients with mild cognitive impairment (MCI) harbor specific signature of bacteria in their gut and that a modified Mediterranean ketogenic diet (MMKD) improves Alzheimer's disease (AD) markers in cerebrospinal fluid (CSF) and the signatures of gut bacteria. However, other microbial population such as gut fungi (mycobiome) in relation to MCI/AD pathology, gut bacteria and diet remain unknown.Methods: We measure gut mycobiome by sequencing of the fungal rRNA ITS1 gene in 17 older adults (11 MCI; 6 cognitively normal [CN]) in a single-center, randomized, double-blind, crossover pilot study, before and after 6 weeks intervention of MMKD and American Heart Association Diet (AHAD), and determine its correlation with AD markers in CSF and gut bacteria.Findings: Compared to CN counterparts, patients with MCI have higher proportion of families Sclerotiniaceae, Phaffomyceteceae, Trichocomaceae, Cystofilobasidiaceae, Togniniaceae and genera Botrytis, Kazachstania, Phaeoacremonium and Cladosporium and lower abundance of Meyerozyma. Specific fungal taxa exhibit distinct correlation arrays with AD markers and gut bacteria in subjects with versus without MCI. MMKD induces broader effect on fungal diversity in subjects with MCI and increases Agaricus and Mrakia while decreasing Saccharomyces and Claviceps with differential response in subjects with or without MCI.Interpretation: The study reveals MCI-specific mycobiome signatures and demonstrates that distinct diets modulate the mycobiome in association with AD markers and fungal-bacterial co-regulation networks in patients with MCI. The findings corroborate the notion of considering gut mycobiome as a unique factor that can affect cognitive health/AD by interacting with gut bacteria and diet and facilitate better understanding of the AD and related microbiome, using unique diet or microbiome modulators. (C) 2020 The Authors. Published by Elsevier B.V.