Interleukin-27 upregulates major histocompatibility complex class II expression in primary human endothelial cells through induction of major histocompatibility complex class II transactivator
Interleukin-27 upregulates major histocompatibility complex class II expression in primary human endothelial cells through induction of major histocompatibility complex class II transactivator
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Interleukin-27 通过诱导主要组织相容性复合物 II 类反式激活因子上调原代人内皮细胞中主要组织相容性复合物 II 类的表达
DOI：
10.1016/j.humimm.2007.10.004
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发表时间：
2007-12-01
期刊：
影响因子：
2.7
通讯作者：
Han, Zhong Chao
中科院分区：
文献类型：
--
作者：
Feng, Xiao Ming;Chen, Xiao Li;Han, Zhong Chao
白细胞介素 - 27（IL - 27）是一种新型的白细胞介素 - 12家族成员，在调节T细胞应答中起关键作用。其对内皮细胞（EC）的免疫调节作用尚未被探究。在此我们展示了IL - 27在诱导原代人内皮细胞中主要组织相容性复合体（MHC）表达方面的作用。IL - 27对人脐静脉内皮细胞的刺激迅速诱导干扰素调节因子 - 1，并显著增加主要组织相容性复合体II类反式激活因子（CIITA）异构体IV的表达。这种反式激活因子的表达与MHC II类基因表达的增加相关。IL - 27还增强MHC I类分子的表达。此外，β2 - 微球蛋白以及与抗原加工相关的转运体 - 1转录本的表达因IL - 27刺激而增加。另外的微阵列分析表明，IL - 27显著上调一组与免疫调节相关的基因，包括人脐静脉内皮细胞中的趋化因子CXCL9、CXCL10和CX3CL1。这首次证明在IL - 27刺激后内皮细胞中MHC II类和I类表达均增加，提示IL - 27在赋予血管内皮免疫功能方面可能很重要。（C）2007年美国组织相容性和免疫遗传学学会。由爱思唯尔公司出版。保留所有权利。
Interleukin-27 (IL-27) is a novel IL-12 family member that plays a critical role in the regulation of T-cell responses. Its immunoregulatory effects on endothelial cells (EC) remain unexplored. Here we show a role for IL-27 in the induction of major histocompatibility complex (MHC) expression in primary human EC. Stimulation of human umbilical vein ECs by IL-27 rapidly induces IFN regulatory factor-1 and dramatically increases the expression of major histocompatibility class II transactivator (CIITA) isoform IV. Expression of this transactivator correlates with increased MHC class II gene expression. IL-27 also enhances expression of MHC class I molecules. Furthermore expression of beta 2-microglobulin and transporter associated with antigen processing-1 transcripts increases in response to IL-27. Additional microarray analysis demonstrates that IL-27 significantly upregulates a panel of genes that correlates with immune regulation, including the chemokines CXCL9, CXCL10, and CX3CL1 in human umbilical vein ECs. This first demonstration that both MHC II and I expression are increased in EC after IL-27 stimulation suggests that IL-27 may be important in conferring immune function on vascular endothelium. (C) 2007 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.