High histamine concentrations in human sweat in association with type I allergy to the semi-purified sweat antigen
High histamine concentrations in human sweat in association with type I allergy to the semi-purified sweat antigen
复制标题
人体汗液中组胺浓度高与对半纯化汗液抗原的 I 型过敏有关
DOI：
10.1016/j.alit.2019.08.011
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发表时间：
2020
影响因子：
6.8
通讯作者：
Hide M
中科院分区：
文献类型：
--
作者：
Takahagi S;Okamoto M;Ishii K;Tanaka A;Yanase Y;Hide M
胆碱能性荨麻疹（CholU）的特征是在出汗条件下，包括热水浴、体育锻炼和情绪压力下，出现针状大小的瘙痒和/或刺痛。1 I型汗液超敏反应可能参与其发病机制。事实上，超过60%的CholU患者在皮内注射自体汗液后表现出立即型皮肤反应，在组胺释放试验（HRA）中，嗜碱性粒细胞对半纯化汗液抗原释放组胺。2,3皮肤肥大细胞释放的组胺在chou的车轮发育中也起重要作用。在这项研究中，我们检测了CholU患者汗液中的组胺浓度，以描述汗液组胺在CholU的轮状形成中的作用。9名CholU患者和10名健康志愿者（对照组）以及7名特应性皮炎（AD）患者和7名慢性自发性荨麻疹（CSU）患者（补充表1）被招募。在进行15 - 30分钟的运动、50℃的桑拿10分钟、热水浴或情绪紧张后，用注射器从受试者的头/颈部或躯干/四肢皮肤收集汗液。采用高效液相色谱法测定汗液/血浆中组胺的含量。使用受试者的嗜碱性粒细胞对半纯化汗液抗原进行HRA反应，如前所述。3本研究获得广岛大学医院机构审查委员会批准（批准号：E-1420）。
Cholinergic urticaria (CholU) is characterized by pinpoint-sized wheals with itching and/or stinging pain under sweating conditions, including hot bathing, physical exercise and emotional stress. 1 The type I hypersensitivity to sweat is proposed to be involved in its pathogenesis. 2 Indeed, more than 60% of patients with CholU shows immediate-type skin reactions upon intradermal injection of autologous sweat, and histamine release from basophils against the semi-purified sweat antigen in histamine release assay (HRA). 2, 3 Histamine released from skin mast cells also plays an important role in the development of wheals in CholU. 1 In this study, we examined histamine concentrations in sweat of patients with CholU to describe a role of sweat histamine in the wheal formation of CholU.Nine patients with CholU and 10 healthy volunteers (control) were recruited along with enrollment of seven patients with atopic dermatitis (AD) and seven patients with chronic spontaneous urticaria (CSU)(Supplementary Table 1). Following exercise for 15e30 min, a sauna at 50 C for 10 min, a bath in hot water or emotional stress, sweat was collected by using a syringe from subjects' skin of the head/neck or the trunk/extremities. The amount of histamine in sweat/plasma was measured by a high-performance liquid chromatography system. 4 HRA in response to the semipurified sweat antigen was performed by using the subjects’ basophils as described previously. 3 This study was approved by the institutional review board of Hiroshima University Hospital (approval number, E-1420).