Transformation of Liquidambar styraciflua using Agrobacterium tumefaciens
Transformation of Liquidambar styraciflua using Agrobacterium tumefaciens
复制标题
利用根癌农杆菌转化枫香
DOI：
10.1007/bf00237423
复制
发表时间：
1993
影响因子：
6.2
通讯作者：
L. M. Lagrimini
中科院分区：
文献类型：
--
作者：
Janet R. Sullivan;L. M. Lagrimini
本文报道了用农杆菌介导的方法对枫香进行分子遗传转化。一种二元TI-质粒载体，其含有赋予对卡那霉素抗性的嵌合新霉素磷酸转移酶基因和嵌合苏云金芽孢杆菌毒素基因、嵌合E. coliβ-glucuronida（GUS），或烟草阴离子过氧化物酶嵌合基因，通过与根癌农杆菌共培养的方法，将其导入枫香。通过基因组DNA印迹或GUS活性的存在，证实在卡那霉素存在下再生的枫香芽被转化。最优化的转化方案和分子转化纳入快速种质改良协议进行了讨论。
We describe the molecular transformation ofLiquidambar styracifluausingAgrobacterium tumefaciens. A binary TI-plasmid vector containing a chimeric neomycin phosphotransferagene which confers resistance to kanamycin and either a chimericBacillus thuringiensistoxin gene, a chimericE. coliβ-glucuronida(GUS), or a chimeric tobacco anionic peroxidase gene was introduced into sweetgum by co-cultivation withAgrobacterium tumefaciens. Sweetgum shoots regenerated in the presence of kanamycin were confirmed to be transformed by genomic DNA blots or the presence of GUS activity. The optimization of the transformation protocol and the incorporation of molecular transformation into a rapid germplasm improvement protocol are discussed.