Postnatal shifts of interneuron position in the neocortex of normal and reeler mice:: Evidence for inward radial migration
Postnatal shifts of interneuron position in the neocortex of normal and reeler mice:: Evidence for inward radial migration
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DOI：
10.1016/j.neuroscience.2003.11.033
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Fink, A
中科院分区：
文献类型：
--
作者：
Hevner, RF;Daza, RAM;Fink, A
在发育过程中，中间神经元通过切向和径向迁移到皮层的精确位置。本研究的目的是描述出生后第一周中间神经元的净径向迁移，并探讨reelin信号在调节这些迁移中的作用。为了观察径向迁移，我们比较了出生后小鼠新皮质中间神经元（对GABA或Dlx有免疫反应）的层状位置(P) 0.5和P7.5。此外，我们使用溴脱氧尿苷出生测定来揭示不同中间神经元群的迁移。为了研究reelin缺乏对小鼠的影响，我们对reelin突变小鼠进行了实验。在正常P0.5皮层，中间神经元在边缘区和第5层最为丰富。到P7.5时，中间神经元在边缘区数量最少，在皮质板中分布更均匀。这种变化主要归因于中后期出生的中间神经元（产生于胚胎日(E) 13.5至E16.5）从边缘区向内迁移到第2-5层。在同一时间间隔内，晚出生的投射神经元（对GABA或Dlx无免疫反应）主要向外迁移，从中间区向皮层上层迁移。在卷叶皮层，中晚出生的中间神经元从P0.5的上板迁移到P7.5的深皮质板。而晚生的投射神经元则相反，从中间区向皮层深部板迁移。我们得出结论，在正常小鼠和reeler小鼠出生后的第一周内，许多中晚期出生的中间神经元径向向内迁移，从边缘区（或上板）到皮质板。我们提出，在皮质板内，神经元间层的位置可能在一定程度上是由与神经发生当天出生的投射神经元的相互作用决定的。(c) 2004 ibro。Elsevier Ltd.出版。版权所有。
During development, interneurons migrate to precise positions in the cortex by tangential and radial migration. The objectives of this study were to characterize the net radial migrations of interneurons during the first postnatal week, and to investigate the role of reelin signaling in regulating those migrations. To observe radial migrations, we compared the laminar positions of interneurons (immunoreactive for GABA or Dlx) in mouse neocortex on postnatal days (P) 0.5 and P7.5. In addition, we used bromodeoxyuridine birthdating to reveal the migrations of different interneuron cohorts. To study the effects of reelin deficiency, experiments were performed in reeler mutant mice.In normal P0.5 cortex, interneurons were most abundant in the marginal zone and layer 5. By P7.5, interneurons were least abundant in the marginal zone, and were distributed more evenly in the cortical plate. This change was attributed mainly to inward migration of middle- to late-born interneurons (produced on embryonic days (E) 13.5 to E16.5) from the marginal zone to layers 2-5. During the same interval, late-born projection neurons (non-immunoreactive for GABA or Dlx) migrated mainly outward, from the intermediate zone to upper cortical layers. In reeler cortex, middle- and late-born interneurons migrated from the superplate on P0.5, to the deep cortical plate on P7.5. Late-born projection neurons in reeler migrated in the opposite direction, from the intermediate zone to the deep cortical plate.We conclude that many middle- and late-born interneurons migrate radially inward, from the marginal zone (or superplate) to the cortical plate, during the first postnatal week in normal and reeler mice. We propose that within the cortical plate, interneuron laminar positions may be determined in part by interactions with projection neurons born on the same day in neurogenesis. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.