Aromatase Is Expressed and Active in the Rainbow Trout Oocyte During Final Oocyte Maturation
Aromatase Is Expressed and Active in the Rainbow Trout Oocyte During Final Oocyte Maturation
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DOI：
10.1002/mrd.21335
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发表时间：
2011-07-01
影响因子：
2.5
通讯作者：
Bobe, Julien
中科院分区：
文献类型：
--
作者：
Gohin, Maella;Bodinier, Pascal;Bobe, Julien
虽然人们普遍认为雌二醇-17 β (E2)合成的卵泡部位仅限于体细胞，但在硬骨鱼中，生发室的可能贡献很少或没有得到关注。为了证实卵巢芳香化酶在卵母细胞中的表达，采用原位杂交技术研究了cyp19a1a mRNA在卵巢卵泡中的表达。此外，利用实时荧光定量PCR技术研究了虹鳟（Oncorhynchus mykiss）卵母细胞最终成熟（即获得成熟能力和随后的减数分裂恢复）过程中卵泡体细胞区室和生发区室cyp19a1a的表达。在卵泡的体细胞室和生发室中也研究了卵巢芳香酶的酶活性。最后，在整个排卵期监测卵泡封闭卵母细胞中的E2水平。我们能够证明一个显着的卵巢芳香酶的表达和活性在卵黄细胞晚期。此外，在卵子形成后期，卵母细胞中芳香化酶的表达和活性急剧下降，同时在周围的卵泡层中观察到这种趋势。我们还报告了在排卵前期间卵母细胞中E2水平的意外增加。据我们所知，这些观察结果在硬骨鱼物种中尚属首次报道。总之，我们的数据支持生发室参与卵泡雌激素合成的假设，以及E2在卵母细胞和/或早期胚胎发育中的生物学作用。摩尔。天线转换开关。开发。78:510- 518,2011。(C) 2011 Wiley-Liss, Inc。
While it is generally well accepted that the ovarian follicular sites of estradiol-17 beta (E2) synthesis are restricted to somatic cells, the possible contribution of the germinal compartment has received little or no attention in teleosts. In order to demonstrate the expression of ovarian aromatase in the oocyte, cyp19a1a mRNA was studied in ovarian follicles by in situ hybridization. In addition, the expression of cyp19a1a was studied in both somatic and germinal compartments of the ovarian follicle in rainbow trout (Oncorhynchus mykiss) during final oocyte maturation (i.e., maturational competence acquisition and subsequent meiosis resumption) by real-time PCR. The enzymatic activity of ovarian aromatase was also studied in both somatic and germinal compartments of the ovarian follicle. Finally, E2 levels were monitored in follicle-enclosed oocytes throughout the pre-ovulatory period. We were able to demonstrate a significant ovarian aromatase expression and activity in the late vitellogenic oocyte. Furthermore, a dramatic decrease in aromatase expression and activity occurs in the oocyte during late oogenesis, concomitantly with the trend observed in surrounding follicular layers. We also report an unexpected increase of E2 levels in the oocyte during the pre-ovulatory period. To our knowledge, these observations are reported for the first time in any teleost species. Together, our data support the hypothesis of the participation of the germinal compartment in follicular estrogen synthesis and a biological role of E2 during oocyte and/or early embryo development. Mol. Reprod. Dev. 78: 510-518, 2011. (C) 2011 Wiley-Liss, Inc.