Serum adipokines in osteoarthritis; comparison with controls and relationship with local parameters of synovial inflammation and cartilage damage
Serum adipokines in osteoarthritis; comparison with controls and relationship with local parameters of synovial inflammation and cartilage damage
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DOI：
10.1016/j.joca.2012.05.002
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发表时间：
2012-08-01
影响因子：
7
通讯作者：
Mastbergen, S. C.
中科院分区：
文献类型：
--
作者：
de Boer, T. N.;van Spil, W. E.;Mastbergen, S. C.
目的：脂肪组织是一种释放脂肪因子的内分泌组织，参与骨关节炎（OA）的发病。然而，它们的相对贡献和确切机制仍然不明确。本研究的目的是比较终末期膝关节OA患者和对照组的血清脂肪因子水平，并将这些血清水平与软骨损伤和滑膜炎症的局部参数联系起来。方法：收集172例重度膝关节OA患者在全膝关节置换术（TKR）前不久的血清，以及132例未进行膝关节OA影像学检查的对照[Kellgren & Lawrence (K&L) = 0]。采用酶联免疫吸附试验（ELISA）测定血清脂联素、瘦素和抵抗素水平。在TKR手术中收集软骨和滑膜组织，通过组织化学和生化分析评估软骨退变和滑膜炎症。结果：OA患者的脂肪因子水平均明显高于对照组。尤其是脂联素和瘦素与女性性别（分别为0.239和0.467,P < 0.001）和体重指数（BMI）（分别为-0.189和0.396,P < 0.001）相关。血清脂肪因子水平与软骨损伤（组织化学，蛋白多糖含量）之间没有相关性，而与滑膜炎症存在弱但正相关性[脂联素和白细胞介素-1 β (IL-1 β)， β值= 0.172,P = 0.02；抵抗素和组织学，β值= 0.183,P = 0.034，经人口统计学调整]。结论：考虑到OA患者的血清水平高于对照组，本研究提示脂肪因子在OA患者中起重要作用。系统性脂肪因子与局部滑膜组织炎症的关联被发现，尽管与软骨损伤没有类似的关系，这表明脂肪因子与OA的炎症成分相关。(C) 2012国际骨关节炎学会。Elsevier Ltd.出版。版权所有。
Objective: Adipose tissue is an endocrine tissue releasing adipokines suggested to be involved in the pathogenesis of osteoarthritis (OA). Nevertheless, their relative contribution and exact mechanisms are still ambiguous. The aim of this study is to compare serum adipokine levels between end-stage knee OA patients and controls and to relate these serum levels to local parameters of cartilage damage and synovial inflammation.Methods: Serum was collected from 172 severe knee OA patients, shortly before total knee replacement (TKR) surgery and from 132 controls without radiographic knee OA [Kellgren & Lawrence (K&L) = 0]. Serum adiponectin, leptin, and resistin levels were measured by enzyme-linked immunosorbent assay (ELISA). Cartilage and synovial tissue were collected at TKR surgery and assessed for cartilage degeneration and synovial inflammation by histochemistry and biochemical analyses.Results: The adipokine levels were all distinctly higher in OA patients as compared to controls. Especially adiponectin and leptin were associated with female gender (stand beta = 0.239 and 0.467, respectively, P < 0.001) and body mass index (BMI) (stand beta = -0.189 and 0.396, respectively, P < 0.001). No associations between serum levels of adipokines and cartilage damage (histochemistry, proteoglycan content) were found whereas weak but positive associations with synovial inflammation were found [adiponectin and interleukin-1 beta (IL-1 beta), stand beta = 0.172, P = 0.02: resistin and histology, stand beta = 0.183, P = 0.034, adjusted for demographics].Conclusion: This study suggests an important involvement of adipokines in OA patients considering their high serum levels compared to controls. Associations of systemic adipokines with local synovial tissue inflammation were found, although not represented by similar relations with cartilage damage, suggesting that adipokines are of relevance in the inflammatory component of OA. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.