Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress
Point mutations in the barley HvHAK1 potassium transporter lead to improved K+-nutrition and enhanced resistance to salt stress
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DOI：
10.1016/j.febslet.2008.10.036
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发表时间：
2008-11-26
期刊：
影响因子：
3.5
通讯作者：
Santa-Maria, Guillermo E.
中科院分区：
文献类型：
--
作者：
Mangano, Silvina;Silberstein, Susana;Santa-Maria, Guillermo E.
I组KT-HAK-KUP转运蛋白的成员在植物根系K+吸收中起重要作用，这一过程受到盐胁迫的强烈影响。对HvHAK 1的基于PCR的随机诱变方法允许鉴定V366 I和R591 C取代，其赋予酵母细胞增强的K+捕获和改善的NaCl、LiCl和NH 4Cl耐受性。改善的K+-捕获与增强的Vmax相关。结果揭示了K+的内在保护作用，并指定一个重要的作用，第8跨膜结构域，以及C-末端，在确定的最大容量的K+运输的KT-HAK-KUP转运蛋白。(C)2008年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Members of group I KT-HAK-KUP transporters play an important role in K+ acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K+-capture, and improved NaCl, LiCl and NH4Cl tolerance, to yeast cells. Improved K+-capture was linked to an enhanced Vmax. Results reveal an intrinsic protective effect of K+, and assign an important role to the 8th transmembrane domain, as well as the C-terminus, in determining the maximum capacity for the transport of K+ in KT-HAK-KUP transporters. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.