Genome-wide identification of FAR gene family and functional analysis of NlFAR10 during embryogenesis in the brown planthopper Nilaparvata lugens.
Genome-wide identification of FAR gene family and functional analysis of NlFAR10 during embryogenesis in the brown planthopper Nilaparvata lugens.
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DOI：
10.1016/j.ijbiomac.2022.11.075
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发表时间：
2022-11
影响因子：
8.2
通讯作者：
Xiaobin Fan;Wenqing Zhang
中科院分区：
文献类型：
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作者：
Xiaobin Fan;Wenqing Zhang
脂肪酰基辅酶A还原酶（法尔斯）催化生物体中相应脂肪酸前体合成脂肪醇。然而，法尔斯在昆虫生殖力和胚胎发生中的功能仍不清楚。本研究共鉴定了22种水稻半翅目害虫褐飞虱（Nilaparvata lugens）的FAR蛋白，其中大部分在胚胎期高表达。其中，NIFAR 10在胚胎发育后期特异性高表达，但在成体组织中混杂表达。异源表达的NIFAR 10能够从相应的酰基-CoA前体产生中间体脂肪酸醇。体内RNAi沉默NIFAR 10基因后，胚胎发生受到明显抑制，孵化率降低。此外，代谢组学分析表明，在胚胎发育过程中，NIFAR 10的损失影响脂质代谢和嘌呤代谢。据我们所知，这是第一个报告的FAR成员影响昆虫胚胎发生，从而为未来的害虫管理提供了一个新的目标。
Fatty acyl-CoA reductases (FARs) catalyze the synthesis of fatty alcohols from corresponding fatty acid precursors in organisms. However, the function of FARs in insect fecundity and embryogenesis remains largely unclear. Here, a total of 22 putative FAR proteins were identified in the brown planthopperNilaparvata lugens, a hemipteran insect pest of rice, and most of them were highly expressed in embryonic stages. Among them, NlFAR10 was specifically and highly expressed in the later embryogenesis, but was promiscuously expressed in tissues of adults. The heterologously expressed NlFAR10 was able to produce the intermediate fatty acid alcohols from the corresponding acyl-CoA precursors. WhenNlFAR10was silenced through RNAi in vivo, the embryogenesis was obviously inhibited, resulting in low hatching rates. Moreover, the metabolome analyses indicated that loss ofNlFAR10affected lipid metabolism and purine metabolism during embryogenesis. To the best of our knowledge, this is the first report of a FAR member affecting insect embryogenesis, thus providing a new target for future pest management.