Association of nitrogen-fixing, plant-growth-promoting rhizobacteria (PGPR) with kallar grass and rice
Association of nitrogen-fixing, plant-growth-promoting rhizobacteria (PGPR) with kallar grass and rice
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DOI：
10.1023/a:1004295714181
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发表时间：
1997-07-01
期刊：
影响因子：
4.9
通讯作者：
Ali, S
中科院分区：
文献类型：
--
作者：
Malik, KA;Bilal, R;Ali, S
褐细叶藓Leptoaca fusca（L.）Kunth（kallar grass）以前曾被发现表现出高固氮率。在营养液和盐渍土中进行了一系列利用N-15同位素稀释法测定生物固氮水平的试验。这些研究表明，土壤中固定的氮素在农学上具有重要意义。卡拉草与水稻有着相似的生长环境。因此，在从根中分离出各种固氮菌后，对水稻进行了类似的研究，这些固氮菌也被筛选出产生生长素（IAA）的能力。在实验室无菌条件下，对NIAB-6和BAS-370两个水稻品种进行了筛选，得到了5个菌株，即Azoacillum lipoferum N-4、Azoacillum brasilense Wb-3、Azoarcus K-1、Pseudomonas 96-51和Zoogloea Ky-1。使用N-15同位素稀释法定量固定的氮。接种固氮菌N-4后，BAS-370的氮素来源接近70%。在微区中使用N-15肥料的混合接种物的类似研究表明，近29%的植物氮来自大气。
Leptochloa fusca (L.) Kunth (kallar grass) has previously been found to exhibit high rates of nitrogen fixation. A series of experiments to determine the level of biological nitrogen fixation using N-15 isotopic dilution were carried out in nutrient solution and saline soil. These studies indicated an agronomically significant amount of nitrogen being fixed in soil. Kallar grass has a similar growth habitat to rice. Therefore similar studies were carried out with rice after isolating various diazotrophs from the roots which were also screened for their ability to produce auxin (IAA). Five such strains namely Azospirillum lipoferum N-4, Azospirillum brasilense Wb-3, Azoarcus K-1, Pseudomonas 96-51, Zoogloea Ky-1 were selected for inoculating two rice varieties i.e. NIAB-6 and BAS-370 under aseptic laboratory conditions. The nitrogen fixed was quantified using the N-15 isotopic dilution method. Variety BAS-370 had nearly 70% nitrogen derived from atmosphere (Ndfa) when inoculated with Azospirillum N-4. Similar studies with the mixed inoculum using N-15 fertilizer in the micro plots indicated that nearly 29% of plant nitrogen was derived from the atmosphere.