Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages
Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages
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DOI：
10.1016/j.jprot.2011.05.008
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发表时间：
2011-09-06
影响因子：
3.3
通讯作者：
Lawrence, Mark L.
中科院分区：
文献类型：
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作者：
Donaldson, Janet R.;Nanduri, Bindu;Lawrence, Mark L.
单核细胞增多性李斯特菌能够在巨噬细胞内存活和增殖。本研究分析了3株单核细胞增多性李斯特菌(血清型1/2a株egde、血清型4b株F2365和血清型4a株HCC23)在小鼠巨噬细胞系J774.1上的增殖能力。我们发现，无毒株HCC23能够引发感染，但不能在巨噬细胞内建立长期感染。相反，菌株egde和F2365在巨噬细胞内增殖至少7h。我们利用多维蛋白质鉴定技术结合电喷雾串联质谱仪，比较了这些菌株在感染后0h、3h和5h的蛋白质表达谱，进一步分析了这些菌株的蛋白质表达谱。我们的结果表明，在感染后3h，三个菌株都表达了相似的代谢和细胞壁相关蛋白。然而，应激反应和DNA修复蛋白的表达增加与感染后5h巨噬细胞的增殖能力有关。通过比较这三株单核细胞增多性李斯特菌在巨噬细胞内生长过程中的蛋白质表达模式，我们能够检测到可能决定单核细胞增多性李斯特氏菌在巨噬细胞中存活能力的生物学差异。(C)2011爱思唯尔B.V.保留所有权利。
Listeria monocytogenes is able to survive and proliferate within macrophages. In the current study, the ability of three L. monocytogenes strains (serovar 1/2a strain EGDe, serovar 4b strain F2365, and serovar 4a strain HCC23) to proliferate in the murine macrophage cell line J774.1 was analyzed. We found that the avirulent strain HCC23 was able to initiate an infection but could not establish prolonged infection within the macrophages. By contrast, strains EGDe and F2365 proliferated within macrophages for at least 7 h. We further analyzed these strains by comparing their protein expression profiles at Oh, 3 h, and 5 h post-infection using multidimensional protein identification technology coupled with electrospray ionization tandem mass spectrometry. Our results indicated that similar metabolic and cell wall associated proteins were expressed by all three strains at 3 h post-infection. However, increased expression of stress response and DNA repair proteins was associated with the ability to proliferate in macrophages at 5 h post-infection. By comparing the protein expression patterns of these three L. monocytogenes strains during intracellular growth in macrophages, we were able to detect biological differences that may determine the ability of L. monocytogenes to survive in macrophages. (C) 2011 Elsevier B.V. All rights reserved.