Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.
Multifaceted Regulation of Gene Expression by the Apoptosis- and Splicing-Associated Protein Complex and Its Components.
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DOI：
10.7150/ijbs.18649
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发表时间：
2017
影响因子：
9.2
通讯作者：
Singh KK
中科院分区：
文献类型：
--
作者：
Deka B;Singh KK
RNA结合蛋白（RBP）在前体mRNA上的差异沉积介导了基因表达的过程。在RNA代谢中起关键作用的含有RBP的复合物之一是剪接相关蛋白（ASAP）复合物。本文就ASAP复合物的结构及其定位作一综述。此外，我们还讨论了不同研究小组对ASAP复合物亚基在RNA代谢中的各种功能的研究结果。ASAP复合物的亚基是RNPS1、Acinus和SAP 18。最初，ASAP复合物被认为将RNA加工与细胞凋亡联系起来。进一步的研究表明，这些组分在细胞RNA代谢中的作用，包括转录、剪接、翻译和无义介导的mRNA衰变（NMD）。在转录中，RNPS1参与阻止R环的形成，而Acinus和SAP18则在组蛋白脱乙酰酶的帮助下抑制转录。一方面，ASAP复合物的单个组分，即RNPS1和腺泡作为剪接激活剂，而另一方面，体外测定显示ASAP复合物作为剪接阻遏物。此外，ASAP复合物的单个成员与外显子连接复合物（EJC）缔合以在剪接和翻译中发挥作用。RNPS1通过参与mRNA的3 '末端加工和多核糖体结合来提高翻译效率。类似地，在NMD期间，RNPS1通过与EJC相互作用来帮助招募衰变因子。
The differential deposition of RNA-binding proteins (RBPs) on pre-mRNA mediates the processes of gene expression. One of the complexes containing RBPs that play a crucial part in RNA metabolism is the apoptosis-and splicing-associated protein (ASAP) complex. In this review, we present a summary of the structure of ASAP complex and its localization. Also, we discuss the findings by different groups on various functions of the subunits of the ASAP complex in RNA metabolism. The subunits of the ASAP complex are RNPS1, Acinus and SAP18. Originally, the ASAP complex was thought to link RNA processing with apoptosis. Further studies have shown the role of these components in RNA metabolism of cells, including transcription, splicing, translation and nonsense-mediated mRNA decay (NMD). In transcription, RNPS1 is involved in preventing the formation of R-loop, while Acinus and SAP18 suppress transcription with the help of histone deacetylase. On the one hand, individual components of the ASAP complex, namely RNPS1 and Acinus act as splicing activators, whereas on the other hand, in-vitro assay shows that the ASAP complex behaves as splicing repressor. In addition, the individual members of the ASAP complex associates with the exon junction complex (EJC) to play roles in splicing and translation. RNPS1 increases the translation efficiency by participating in the 3'end processing and polysome association of mRNAs. Similarly, during NMD RNPS1 aids in the recruitment of decay factors by interacting with EJC.