Organic and inorganic fertilizers respectively drive bacterial and fungal community compositions in a fluvo-aquic soil in northern China
Organic and inorganic fertilizers respectively drive bacterial and fungal community compositions in a fluvo-aquic soil in northern China
复制标题
有机和无机肥料分别驱动中国北方潮土中的细菌和真菌群落组成
DOI：
10.1016/j.still.2019.104540
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发表时间：
2020-04-01
影响因子：
6.5
通讯作者：
Zhuge, Yuping
中科院分区：
文献类型：
--
作者：
Pan, Hong;Chen, Manman;Zhuge, Yuping
土壤微生物在能量流动和养分循环中起着至关重要的作用，因此对农业生产具有重要意义。更好地了解微生物群落对各种有机和无机施肥制度的复杂反应对于农业生态系统的可持续发展至关重要。研究了北方潮土在38年长期施肥措施下细菌和真菌丰度和多样性的变化。通过高通量测序和基于定量聚合酶链反应的细菌16S rRNA和真菌内部转录间隔区（ITS）rRNA基因扩增，研究了不同施肥处理（N、NP或NPK）、有或没有施用粪肥改良剂的土壤中细菌16S rRNA和真菌内部转录间隔区（ITS）rRNA基因的变化。化肥配施有机肥明显提高了土壤肥力，可作为进一步优化施肥模式的参考。主成分分析和偏最小二乘判别分析均表明，有机肥对细菌群落分布的影响大于化肥，而真菌群落对无机肥的影响更敏感。线性判别分析效应量法表明，施用有机肥处理的土壤细菌群落中响应微生物（受不同施肥影响显著的微生物）数量显著高于施用化肥处理的土壤，而真菌群落中响应微生物数量则呈现相反的趋势。此外，冗余度分析进一步说明了有机质在塑造细菌群落分布中的重要性，而不是在驱动真菌群落模式。这些结果表明，有机肥和无机肥，分别占主导地位的塑造细菌和真菌群落分布在潮土。
Soil microbes play vital roles in energy flow and nutrient cycling and, thus, are important for agricultural production. A better understanding of the complex responses of microbial communities to various organic and inorganic fertilization regimes is critical for sustainable development of agroecosystems. Changes in bacterial and fungal abundance and diversity in fluvo-aquic soil in Northern China were studied under 38-year long-term fertilization strategies: four chemical-fertilization strategies (i.e., no fertilizer, N, NP, or NPK), with or without manure amendment, were investigated by high-throughput sequencing and quantitative polymerase chain reaction-based amplification of bacterial 16S rRNA and fungal internal transcribed spacer (ITS) rRNA genes. Chemical fertilizer plus manure addition clearly increased the soil fertility and was recommended for further optimization of fertilization patterns. Both principal component analysis and partial least-square discriminant analysis showed greater impacts of manure addition than chemical fertilizer on bacterial community distributions, whereas fungal communities were more sensitive to inorganic fertilizer. The linear discriminant analysis effect size method revealed that the number of responding microbes (microbes significantly affected by various fertilizations) in bacterial communities in manure-treated soils was markedly higher than that in chemical fertilizer-treated soils, whereas those of fungal communities showed the opposite trend. In addition, redundancy analysis further illustrated the primary importance of organic matter in shaping community distributions of bacteria, rather than in driving fungal community patterns. These results suggested that organic and inorganic fertilizers, respectively, dominated in shaping bacterial and fungal community distributions in fluvo-aquic soils.