ANTAGONISM OF THE EFFECTS OF CALCITONIN GENE-RELATED PEPTIDE AND OF CAPSAICIN ON THE GUINEA-PIG ISOLATED ILEUM BY HUMAN ALPHA-CALCITONIN GENE-RELATED PEPTIDE(8-37)
ANTAGONISM OF THE EFFECTS OF CALCITONIN GENE-RELATED PEPTIDE AND OF CAPSAICIN ON THE GUINEA-PIG ISOLATED ILEUM BY HUMAN ALPHA-CALCITONIN GENE-RELATED PEPTIDE(8-37)
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DOI：
10.1016/0304-3940(91)90744-e
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发表时间：
1991-08-05
影响因子：
2.5
通讯作者：
SZOLCSANYI, J
中科院分区：
文献类型：
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作者：
BARTHO, L;KOCZAN, G;SZOLCSANYI, J
用降钙素基因相关肽（CGRP）拮抗剂hCGRP（8-37）研究了CGRP在辣椒素作用于豚鼠回肠中的可能介导作用。 由大鼠CGRP（3 nM）或辣椒素（1 μ M）引起的组胺预收缩回肠的次最大纵向肌肉松弛被hCGRP（8-37）（1.5 μ M）逆转，而肾上腺素或神经降压素引起的纵向肌肉松弛不受影响。 辣椒素（100 nM）对自发性循环肌肉运动的抑制作用也被hCGRP逆转（8-37）。 CGRP拮抗剂对电刺激诱发的回肠收缩或纵向或环形肌张力没有影响。 结论：（a）hCGRP（8-37）是回肠中的一种特异性CGRP拮抗剂，显然没有内在活性或任何与CGRP无关的作用;（B）辣椒素对回肠纵肌和环肌的抑制作用至少部分是由CGRP介导的。
The possible mediating role of calcitonin gene-related peptide (CGRP) in the effects of capsaicin in the guinea-pig ileum has been investigated by means of the CGRP antagonist hCGRP(8-37). Submaximal longitudinal muscle relaxation of the histamine-precontracted ileum evoked by rat CGRP (3 nM) or capsaicin (1-mu-M) was reversed by hCGRP(8-37) (1.5-mu-M), while that due to adrenaline or neurotensin was not affected. Inhibition of spontaneous circular muscle movements by capsaicin (100 nM) was also reversed by hCGRP(8-37). The CGRP antagonist had no effect on electrical stimulation-evoked ileal contractions or on the longitudinal or circular muscle tone. It is concluded that (a) hCGRP(8-37) is a specific CGRP antagonist in the ileum, apparently devoid of intrinsic activity or any effect not related to CGRP; (b) the inhibitory actions of capsaicin on the longitudinal and circular muscles of the ileum are mediated, at least in part, by CGRP.