Antimicrobial susceptibility of Neisseria gonorrhoeae isolates from Hefei (2014–2015): genetic characteristics of antimicrobial resistance
Antimicrobial susceptibility of Neisseria gonorrhoeae isolates from Hefei (2014–2015): genetic characteristics of antimicrobial resistance
复制标题
合肥地区淋病奈瑟菌耐药性分析（2014-2015）：耐药性遗传特征
作者：
F. Jiang;Qian Lan;W. Le;X. Su
背景耐药性（antimicrobial resistance，AMR）及耐药基因的研究进展对合肥地区126株淋球菌进行了分子流行病学研究，为我国淋球菌耐药性的分子流行病学研究提供了新的信息。2014年1月至2015年11月期间，从合肥一家医院门诊收集淋病分离株。测定了N.用琼脂稀释法测定淋球菌分离株对7种抗菌剂的敏感性。检测分离株的佩纳和mtrR基因以及23 S rRNA突变，并使用N.淋球菌多抗原序列分型（NG-MAST）。淋球菌对环丙沙星、四环素和青霉素的耐药率分别为81.7%（103/126）和73.8%（93/126）。39.7%（50/126）的菌株为产青霉素酶N.淋病奈瑟菌（PPNG），31.7%（40/126）耐四环素。对阿奇霉素耐药率为28.6%（36/126）。14株（11.1%）菌株对头孢曲松（MIC ≥ 0.125 mg/L，n = 10）、头孢克肟（MIC ≥ 0.125 mg/L，n = 10）、头孢噻肟（MIC ≥ 0.125 mg/L，n = 10）敏感性降低。25 mg/L，n = 1）或两个ESC（n = 3）。佩纳嵌合等位基因XXXV被发现在所有的菌株，窝藏头孢克肟的敏感性下降，除了一个。在mtrR基因中发现了4个突变，在23 SrRNA中发现了A2143 G和C2599 T突变。没有分离株对壮观霉素耐药。淋球菌分离株分布于不同的NG-MAST序列类型（ST）;共鉴定出86个独立的ST。2014-2015年期间合肥的淋病分离株对先前推荐用于治疗淋病的抗菌药物表现出高水平的耐药性，例如，青霉素四环素和环丙沙星阿奇霉素耐药率也很高（28.6%）。没有发现分离株对壮观霉素、头孢曲松或头孢克肟完全耐药;然而，总体而言，11.1%的分离株对ESC的敏感性降低。
BackgroundAntimicrobial resistance (AMR) and genetic determinants of resistance of N. gonorrhoeae isolates from Hefei, China, were characterized adding a breadth of information to the molecular epidemiology of gonococcal resistance in China.Methods126 N. gonorrhoeae isolates from a hospital clinic in Hefei, were collected between January, 2014, and November, 2015. The minimum inhibitory concentration (MIC) of N. gonorrhoeae isolates for seven antimicrobials were determined by the agar dilution method. Isolates were tested for mutations in penA and mtrR genes and 23S rRNA, and also genotyped using N. gonorrhoeae multi-antigen sequence typing (NG-MAST).ResultsAll N. gonorrhoeae isolates were resistant to ciprofloxacin; 81.7% (103/126) to tetracycline and 73.8% (93/126) to penicillin. 39.7% (50/126) of isolates were penicillinase producing N. gonorrhoeae (PPNG), 31.7% (40/126) were tetracycline resistant N. gonorrhoeae (TRNG) and 28.6% (36/126) were resistant to azithromycin. While not fully resistant to extended spectrum cephalosporins (ESCs), a total of 14 isolates (11.1%) displayed decreased susceptibility to ceftriaxone (MIC ≥ 0.125 mg/L, n = 10), cefixime (MIC ≥ 0. 25 mg/L, n = 1) or to both ESCs (n = 3). penA mosaic alleles XXXV were found in all isolates that harbored decreased susceptibility to cefixime, except for one. Four mutations were found in mtrR genes and mutations A2143G and C2599T were identified in 23S rRNA. No isolates were resistant to spectinomycin. Gonococcal isolates were distributed into diverse NG-MAST sequence types (STs); 86 separate STs were identified.ConclusionsN. gonorrhoeae isolates from Hefei during 2014–2015, displayed high levels of resistance to antimicrobials that had been recommended previously for treatment of gonorrhea, e.g., penicillin, tetracycline and ciprofloxacin. The prevalence of resistance to azithromycin was also high (28.6%). No isolates were found to be fully resistant to spectinomycin, ceftriaxone or cefixime; however, 11.1% isolates, overall, had decreased susceptibility to ESCs.
影响因子：
3.2
作者：
Barry PM;Klausner JD
通讯作者：
Klausner JD