Analysis and modeling of the primary cilium bending response to fluid shear
Analysis and modeling of the primary cilium bending response to fluid shear
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DOI：
10.1152/ajprenal.1997.272.1.f132
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发表时间：
1997-01-01
影响因子：
4.2
通讯作者：
Bowser, SS
中科院分区：
文献类型：
--
作者：
Schwartz, EA;Leonard, ML;Bowser, SS
由于大多数哺乳动物肾上皮细胞的原生(9+0)纤毛不活动，投射到管腔中，使其暴露在流体运动中，因此本研究观察了初级纤毛对流体剪应力的反应。在流体剪切力(10(-11)-10(-10)N)作用下，初级纤毛发生可逆的大角度弯曲。M(2)=10(-8)-10(-7)dyn/cm)在体外用PtK1细胞的视频显微镜侧视进行表征，然后将纤毛数学模型化为悬臂梁。计算出初级纤毛的弯曲刚度为3.1+/-0.8×10(-23)N。M(2)，修正的四重积分方法，1.4-1.6×10(-23)N。M(2)与‘’重弹性‘’理论。将理论曲线与实验纤毛弯曲响应进行比较，证实了“Heavy Elastica”模型的有效性；该模型又被用来预测大鼠肾单位典型条件下的初级纤毛弯曲行为。这项研究的结果与初级纤毛在肾上皮细胞中起机械感觉功能的假设是一致的。
Since a nonmotile, primary(9 + 0) cilium projects from most mammalian kidney epithelial cells into the tubule lumen, where it is exposed to fluid motion, the present study examined primary cilium response to fluid shear stress. The reversible, large-angle bending of the primary cilium upon exposure to fluid shear forces (10(-11)-10(-10) N . m(2) = 10(-8)-10(-7) dyn/cm) was characterized in vitro using videomicroscopic side views of PtK1 cells, and the cilium was then mathematically modeled as a cantilevered beam. The flexural rigidity of the primary cilium was calculated to be 3.1 +/- 0.8 x 10(-23) N . m(2) with a corrected quadruple integration approach and 1.4-1.6 x 10(-23) N . m(2) with the ''heavy elastica'' theory. Comparison of theoretical profiles to the experimental bending responses of cilia established the validity of the ''heavy elastica'' model; this model, in turn, was used to predict primary cilium bending behavior under representative conditions in the rat nephron. The results of the study are consistent with the hypothesis that primary cilia serve a mechanosensory function in kidney epithelial cells.