HEMODYNAMIC-CHANGES AND COMPENSATORY MECHANISMS DURING EARLY CARDIOGENESIS AFTER NEURAL CREST ABLATION IN CHICK-EMBRYOS
HEMODYNAMIC-CHANGES AND COMPENSATORY MECHANISMS DURING EARLY CARDIOGENESIS AFTER NEURAL CREST ABLATION IN CHICK-EMBRYOS
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DOI：
10.1203/00006450-199112000-00002
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发表时间：
1991-12-01
影响因子：
3.6
通讯作者：
KIRBY, ML
中科院分区：
文献类型：
--
作者：
LEATHERBURY, L;CONNUCK, DM;KIRBY, ML
显微电影摄影术被用来研究鸡胚的早期心脏发育与消融的神经嵴已知导致持续动脉干与相关的主动脉弓异常。 切除第三和第四咽弓的前迁移神经嵴和咽隔。 当胚胎达到环状心管阶段（阶段18）时，在受控环境条件下以100帧/秒拍摄15个实验胚胎和15个对照胚胎。 舒张末期和收缩末期的尺寸确定圆锥干和推定的右心室，共同组成的球心。 结果表明，实验胚胎的缩短率和射血分数明显降低。 实验胚胎表现出心室扩张和排空减少。 对照组和实验组胚胎的心率和每搏输出量没有差异。 因此，计算的心输出量在对照组和实验组中相同。 实验胚胎似乎通过心室扩张来补偿收缩力的下降。 这些功能性代偿在非常早期的心脏发育中可能在随后的结构性心脏缺陷的发展中发挥病因学作用。
Microcinephotography was used to study early heart development in chick embryos with ablations of the neural crest known to result in persistent truncus arteriosus with associated aortic arch anomalies. The premigratory neural crest destined for the 3rd and 4th pharyngeal arches and the aorticopulmonary septum were ablated. When the embryos reached the looped cardiac tube stage (stage 18), 15 experimental, and 15 control embryos were filmed at 100 frames/s under controlled environmental conditions. End-diastolic and end-systolic dimensions were determined for the conotruncus and presumptive right ventricle that together compose the bulbus cordis. The results showed that the shortening fractions and ejection fractions were significantly depressed in the experimental embryos. The experimental embryos exhibited dilation and decreased emptying of the ventricle. There was no difference in heart rate or stroke volume between the control and experimental embryos. Thus, the calculated cardiac output was the same in the control and experimental groups. It appeared that the experimental embryos compensated for decreased contractility by ventricular dilation. These functional compensations in very early cardiac development may play an etiologic role in the subsequent development of structural heart defects.