Dynamic distribution of TTK in HeLa cells: insights from an ultrastructural study
Dynamic distribution of TTK in HeLa cells: insights from an ultrastructural study
复制标题
DOI：
10.1038/sj.cr.7290186
复制
发表时间：
2003-12-01
期刊：
影响因子：
44.1
通讯作者：
Yao, XB
中科院分区：
文献类型：
--
作者：
Dou, Z;Sawagechi, A;Yao, XB
进入有丝分裂是由有丝分裂激酶的信号级联驱动的。我们最近的研究表明TTK是一种与着丝点相关的蛋白激酶，它与CENP-E相互作用，CENP-E是一种位于着丝点冠纤维上的有丝分裂激酶。利用免疫电镜，我们发现TTK存在于HeLa间期细胞的核孔邻近复合体中。在核膜断裂时，TTK以单取向染色体发育中的着丝点的最外层区域以及纺锤极为目标。在稳定附着后，在整个染色体发育过程中，TTK是冠状纤维的一个组成部分，从着丝点外板延伸至90nm处。在中期比对时，TTK离开着丝点并向中心体迁移。综上所述，这一证据有力地支持了TTK在纺锤体和着丝体的纺锤体检查点信号级联中起作用的模型。
Entry into mitosis is driven by signaling cascades of mitotic kinases. Our recent studies show that TTK, a kinetochore-associated protein kinase, interacts with CENP-E, a mitotic kinesin located to corona fiber of kinetochore. Using immunoelectron microscopy, here we show that TTK is present at the nuclear pore adjacent complex of interphase HeLa cells. Upon nuclear envelope fragmentation, TTK targets to the outermost region of the developing kinetochores of monoorient chromosome as well as to spindle poles. After stable attachment, throughout chromosome congression, TTK is a constituent of the corona fibers, extending up to 90 nm away from the kinetochore outer plate. Upon metaphase alignment, TTK departs from the kinetochore and migrates toward the centrosomes. Taken together, this evidence strongly supports a model in which TTK functions in spindle checkpoint signaling cascades at both kinetochore and centrosome.