Precise Tumor Resection under the Navigation of Tumor-Microenvironment pH-activated NIR-II Fluorescence Imaging via Calcium Carbonate/Polydopamine Co-packed Nd-doped Downshifting Nanoprobes
Precise Tumor Resection under the Navigation of Tumor-Microenvironment pH-activated NIR-II Fluorescence Imaging via Calcium Carbonate/Polydopamine Co-packed Nd-doped Downshifting Nanoprobes
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DOI：
10.1016/j.matdes.2023.111703
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发表时间：
2023-02
期刊：
影响因子：
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通讯作者：
Jiaqi Li;Kangliang Lou;Yongying Dang;Haina Tian;Qiang Luo;Cailin Huang;Rongli Liu;Xiangwen Gong;Shifeng Wang;Hui Liu;Peiyuan Wang;Xiaolong Liu
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作者：
Jiaqi Li;Kangliang Lou;Yongying Dang;Haina Tian;Qiang Luo;Cailin Huang;Rongli Liu;Xiangwen Gong;Shifeng Wang;Hui Liu;Peiyuan Wang;Xiaolong Liu
具有第二近红外区域（NIR-II，1000-1700 nm）荧光的Nd掺杂纳米晶体的抗光漂白性、高量子产率和深层组织成像占据了精确导航肿瘤切除的不可或缺的位置。然而，这些NIR-II纳米探针经常在网状内皮系统（RES）器官中遇到高的被动积累，其减弱了信号与背景比（SBR），导致其应用于准确的肿瘤轮廓描绘的障碍。在此，空心结构的碳酸钙和聚多巴胺共包装壳已被共包覆在Nd掺杂的下移纳米晶体（DSNCs@hPCa）上。在中空表面（DSNCs@hPCa-FA）上缀合叶酸分子后，这种迷人的NIR-II造影剂可以进一步特异性地增强肿瘤靶向能力，并令人满意地抑制“OFF”状态下的NIR-II荧光。它敏感地响应于弱酸性肿瘤微环境，因此，混合纳米壳被降解，并且NIR-II荧光被有效地打开。增强SBR的稳健荧光成像已被证明可以准确识别正常组织的肿瘤边界。在这种新的pH刺激响应NIR-II荧光成像的导航下彻底切除肿瘤，并在术后28天后发现原位复发或转移。该策略表明肿瘤微环境激活的纳米探针用于辅助准确描绘肿瘤边缘的临床前潜力。
Photobleaching resistance, high quantum yield and deep tissue imaging of Nd-doped nanocrystals with fluorescence in the second near infrared region (NIR-II, 1000–1700 nm) occupied an indispensable position for precisely navigating tumor resection. However, these NIR-II nanoprobes often encounter high passive accumulation in reticuloendothelial system (RES) organs that attenuates signal to background ratio (SBR), resulting in the impediment of their application for accurate tumor outline delineation. Herein, a hollow-structured CaCO3and polydopamine co-packed shell has been co-cladded on the Nd-doped down-shifting nanocrystals (DSNCs@hPCa). After conjugating folic acid molecules on the hollowed surface (DSNCs@hPCa-FA), this fascinating NIR-II contrast nanoagent can further specifically enhance tumor targeting capacity and gratifyingly restrain the NIR-II fluorescence in the “OFF” state. It sensitively responds to the weak acid tumor microenvironment, consequently, the hybrid nanoshell is degraded and the NIR-II fluorescence is efficiently turn “ON”. Robust fluorescent imaging with the augmented SBR has been demonstrated to accurately identify tumor borderlines from normal tissue. Tumors were thoroughly removed under the navigation of this novel pH stimuli-responsive NIR-II fluorescence imaging with ignorablein siturecurrence or metastases after 28 days post-surgery. This strategy indicates the preclinical potential of tumor microenvironment activated nanoprobes for assisting the accurate delineation of tumor margins.