Plasma kinetics in man of epicatechin from black chocolate
Plasma kinetics in man of epicatechin from black chocolate
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DOI：
10.1038/sj.ejcn.1600673
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发表时间：
1999-01-01
影响因子：
4.7
通讯作者：
Offord, EA
中科院分区：
文献类型：
--
作者：
Richelle, M;Tavazzi, I;Offord, EA
目的：评价黑巧克力中表儿茶素在人体内的血药动力学。每个人都是自己的对照。可可碱被用作巧克力摄入量的对照标记物。地点：代谢部门，雀巢研究中心，Vers-chez-les-Blanc，瑞士。受试者：8名健康男性志愿者（4名吸烟者和4名不吸烟者）入组本研究。他们从富含多酚的食物（咖啡，茶，葡萄酒，果汁，可可产品）的24小时之前，测试，直到其完成。干预：志愿者吃了40克和80克的黑巧克力（雀巢黑）与面包，一个星期的间隔。在食用巧克力后的前4小时内每小时抽取一次血样，最后一次在8小时抽取。结果：食用巧克力后，血浆中表儿茶素和可可碱浓度显著升高（P = 0.002和P = 0.001），在2 ~ 3 h达到最大值。两种底物的血浆动力学曲线下的最大浓度和面积与巧克力的剂量非常相关。结论：表儿茶素从巧克力中吸收，并迅速从血浆中消除。80 g黑巧克力的血浆中的ATP值为0.7 μ mol/l。
Objective: To evaluate the plasma kinetics in man of epicatechin from black chocolate.Design: An intervention study with 8 volunteers. Each served as his own control. Theobromine was used as control marker of the chocolate intake.Setting: Metabolic Unit, Nestle Research Center, Vers-chez-les-Blanc, Switzerland. Subjects: Eight healthy male volunteers (4 smokers and 4 non-smokers) were enrolled in this study. They abstained from foods rich in polyphenols (coffee, tea, wine, fruit juice, cocoa products) for 24 h prior to the test until its completion.Intervention: Volunteers ate 40 g and 80 g of black chocolate (Nestle Noir) together with bread with a one-week interval. Blood samples were drawn every hour during the first 4 h and a last one at 8 h after chocolate consumption. Plasma samples were analysed for epicatechin and theobromine content by HPLC.Results: Plasma concentrations of epicatechin and theobromine increased markedly after chocolate consumption (P = 0.002 and P = 0.001, respectively), reaching a maximum between 2 and 3 h. The maximal concentration and area under the curve of plasma kinetics of both substrates correlated very well with the dose of chocolate.Conclusions: Epicatechin is absorbed from chocolate and is rapidly eliminated from plasma. Attainable plasma values are 0.7 mu mol/l from 80 g of black chocolate.