Assessing porcine liver-derived biomatrix for hepatic tissue engineering
Assessing porcine liver-derived biomatrix for hepatic tissue engineering
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DOI：
10.1089/ten.2004.10.1046
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发表时间：
2004-07-01
影响因子：
--
通讯作者：
Bhatia, SN
中科院分区：
文献类型：
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作者：
Lin, P;Chan, WCW;Bhatia, SN
脱细胞生物衍生基质如小肠粘膜下层已被广泛用于诱导结缔组织、血管移植物和膀胱的组织再生和重塑;然而，脱细胞支架尚未被探索其在肝组织工程中的潜在效用。在基于体外肝细胞的装置和可植入肝细胞支架组织工程构建体的情况下，维持肝细胞功能是最重要的。在这项研究中，我们专门探讨了脱细胞，猪肝衍生的生物基质（LBM）作为原代肝细胞的生物可吸收支架。原代大鼠肝细胞培养LBM和比较，以及表征肝细胞培养模型双凝胶文化，促进维持肝脏特异性功能的许多周，和吸附的胶原蛋白单层，导致肝细胞功能和活力的快速下降。肝细胞在LBM上维持长达45天，发现肝脏特异性功能如白蛋白合成、尿素产生和P-450 IA 1活性比吸附胶原培养物显著改善。我们的数据表明，LBM可能是一个有利的替代现有的组织工程支架，因为它是生物可吸收的，可以很容易地操作，并支持长期的肝细胞功能在体外。
Acellular, biologically derived matrices such as small intestinal submucosa have been extensively utilized to induce tissue regeneration and remodeling of connective tissue, vascular grafts, and urinary bladder; however, decellularized scaffolds have not been explored for their potential utility in hepatic tissue engineering. In the case of both extracorporeal hepatocyte-based devices and implantable hepatocyte-scaffold tissue-engineered constructs, maintenance of hepatocellular function is of prime importance. In this study, we specifically explored decellularized, porcine, liver-derived biomatrix (LBM) as a bioresorbable scaffold for primary hepatocytes. Primary rat hepatocytes were cultured on LBM and compared with well-characterized hepatocyte culture models-double-gel cultures that promote maintenance of fiver-specific functions for many weeks, and adsorbed collagen monolayers that lead to the rapid decline of hepatocellular function and viability. Hepatocytes were maintained for up to 45 days on LBM and liver-specific functions such as albumin synthesis, urea production, and P-450 IA1 activity were found to be significantly improved over adsorbed collagen cultures. Our data indicate that LBM may be a favorable alternative to existing scaffolds for tissue engineering in that it is bioresorbable, can be easily manipulated, and supports long-term hepatocellular functions in vitro.