DEMONSTRATION OF CELLULAR UPTAKE OF POLYMERIZED DNA IN MAMMALIAN CELL CULTURES
DEMONSTRATION OF CELLULAR UPTAKE OF POLYMERIZED DNA IN MAMMALIAN CELL CULTURES
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DOI：
10.1016/0006-291x(60)90206-0
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发表时间：
1960-01-01
影响因子：
3.1
通讯作者：
GARTLER, SM
中科院分区：
文献类型：
--
作者：
GARTLER, SM
结果 图 1 和图 2 显示了两次双标记 DNA 摄取实验的结果。每幅图中上方的曲线是双标记供体DNA的密度梯度分析，下方的曲线显示摄取实验的密度梯度分析。在下面的曲线中，在每个实验中都可见单个 260 v 吸收峰，其密度 posFtFon 等于未标记的宿主细胞 DNA 的密度。可以看到两个小的放射性峰，较大的一个峰的密度等于实验中使用的双标记 DNA 的密度，较小的一个峰与主 260 mp 峰重合。放射性源自供体 DNA，总吸收量约为 0.04 pg。和 0.03 皮克。分别在两个实验中。这大约是 0.57。在每个实验中，所施用的供体DNA的3.0%以及宿主DNA的大约0.1%和0.9%。 129
Results In figures 1 and 2 are shown the results of two uptake experiments with doubly labeled DNA. The upper curve in each figure is the density gradient analysis of the doubly labeled donor DNA, while the lower curves show the density gradient analyses of the uptake experiments. In the lower curve, a single 260 vabsorbing peak is visible in each of the experiments at a density posFtFon equal to that of unlabeled host cell DNA. Two small radioactive peaks are visible, the larger one at a density equal to that of the doubly labeled DNA used in the experiment, and the smaller one coincident with the main 260 mp peak. The radioactivity is derived from donor DNA and represents a total uptake of approximately 0.04 pg. and 0.03 pg. in the two experiments respectively. This is approximately 0.57. and 3.0% of the administered donor DNA and approximately 0.1% and 0.9% of ehe host DNA in each experiment. 129