Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture
Investigating the Interaction of Cellulose Nanofibers Derived from Cotton with a Sophisticated 3D Human Lung Cell Coculture
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DOI：
10.1021/bm200865j
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发表时间：
2011-10-01
影响因子：
6.2
通讯作者：
Weder, Christoph
中科院分区：
文献类型：
--
作者：
Clift, Martin J. D.;Foster, E. Johan;Weder, Christoph
纤维素纳米纤维是多种纳米材料中极具吸引力的成分。它们令人着迷的机械性能和低成本以及纤维素的可再生性质使它们成为碳纳米管（CNT）的有吸引力的替代品，而碳纳米管在吸入时可能会造成相当大的健康风险。然而，人们对气雾化纤维素纳米纤维的潜在毒性知之甚少。使用人上皮气道屏障的 3D 体外三细胞共培养模型，观察到从棉花中分离的纤维素纳米纤维 (CCN) 比多壁碳纳米管 (MWCNTS) 和青石棉纤维 (CAF) 引起的细胞毒性和（促）炎症反应显着降低 (p < 0.05)。电子断层扫描分析还显示，CCN 的细胞内定位与 MWCNT 和 CAF 的细胞内定位不同，表明每种不同纳米纤维类型在与人肺细胞共培养物相互作用方面存在根本差异。因此，本研究中显示的数据强调，不仅长度和刚度决定任何纳米纤维的潜在有害（生物）影响，而且所使用的材料可以显着影响纳米纤维与细胞的相互作用
Cellulose nanofibers are an attractive component of a broad range of nanomaterials. Their intriguing mechanical properties and low cost, as well as the renewable nature of cellulose make them an appealing alternative to carbon nanotubes (CNTs), which may pose a considerable health risk when inhaled. Little is known, however, concerning the potential toxicity of aerosolized cellulose nanofibers. Using a 3D in vitro triple cell coculturer model of the human epithelial airway barrier, it was observed that cellulose nanofibers isolated from cotton (CCN) elicited a Significantly (p < 0.05) lower cytotoxicity and (pro-)inflammatory response than multiwalled CNTs (MWCNTS) and crocidolite asbestos fibers (CAFs). Electron tomography analysis also revealed that the intracellular localization of CCNs is different from that of both MWCNTs and CAFs, indicating fundamental differences between each different nanofibre type in their interaction with the human lung cell coculture. Thus, the data shown in the present study highlights that not only the length and stiffness determine the potential detrimental (biological) effects of any nanofiber, but that the material used can significantly affect nanofiber-cell interactions