Quantitative Proton Nuclear Magnetic Resonance evaluation and total assignment of the capsular polysaccharide Neisseria meningitidis serogroup X
Quantitative Proton Nuclear Magnetic Resonance evaluation and total assignment of the capsular polysaccharide Neisseria meningitidis serogroup X
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DOI：
10.1016/j.jpba.2012.07.014
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Velez, Herman
中科院分区：
文献类型：
--
作者：
Garrido, Raine;Puyada, Alain;Velez, Herman
脑膜炎奈瑟菌是婴儿脑膜炎球菌病的主要病因。血清型A、B、C、W135、V、X在儿童和青少年中的发病率较高。使用多价结合碳水化合物为基础的疫苗已经减少了世界各地的脑膜炎球菌感染。最近，已发现血清群X负责不同的脑膜炎球菌疾病的爆发，主要是在非洲的“脑膜炎带”，并且已通过一维C-13 NMR研究证实了荚膜多糖的重复单元的结构。没有进行进一步的表征研究，特别是使用其他核。本文提出了一种新的N.报道了通过质子qNMR测定脑膜炎血清群X的方法。还通过结合涉及C-13、H-1和P-31核的相关性实验对血清群X多糖进行了深入表征。(C)2012爱思唯尔有限公司版权所有。
Neisseria meningitidis constitutes the main cause of meningococcal disease in infants. Serogroups A, B, C, W135, V. and X have the higher incidence in young children and teenagers. The use of polyvalent conjugate carbohydrate-based vaccines has decreased the meningococcal infection around the world. Recently, the serogroup X has been found to be responsible of different outbreaks of meningococcal diseases, mainly in "Meningitis Belt" of Africa and the structure of the repetitive unit of the capsular polysaccharide has been confirmed through a monodimensional C-13 NMR study. No further characterization studies have been carried out, especially with the use of other nuclei. In this paper a novel method for quantification of the N. meningitidis serogroup X by proton qNMR is reported. Deep characterization of the serogroup X polysaccharide was also carried out by combination of correlation experiments involving C-13, H-1, and P-31 nuclei. (C) 2012 Elsevier B.V. All rights reserved.