Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions
Lamellipodia mediate the heterogeneity of central olfactory ensheathing cell interactions
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DOI：
10.1007/s00018-010-0280-3
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发表时间：
2010-05-01
影响因子：
8
通讯作者：
St John, James A.
中科院分区：
文献类型：
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作者：
Windus, Louisa C. E.;Lineburg, Katie E.;St John, James A.
初级嗅轴突的生长和引导部分归因于嗅鞘细胞（OECs）的存在。然而，很少有人了解的差异OECs的亚群和什么调节他们的相互作用。我们使用嗅鞘细胞轴突测定，并确定轴突不同的周边和中央嗅鞘细胞的反应。然后，我们进一步纯化嗅鞘细胞从解剖学上不同的区域的嗅球。细胞行为分析表明，嗅鞘细胞从嗅球是一个功能异质性的人口与明显的差异，这是一致的，他们提出的作用在体内。我们发现，异质性调节运动lamellipodial波沿着轴的嗅鞘细胞和抑制lamellipodial波活动通过Mek 1废除的能力的细胞，以区分彼此。这些结果表明，嗅鞘细胞从嗅球是一个异质性的人口，使用板状波来调节细胞-细胞识别。
The growth and guidance of primary olfactory axons are partly attributed to the presence of olfactory ensheathing cells (OECs). However, little is understood about the differences between the subpopulations of OECs and what regulates their interactions. We used OEC-axon assays and determined that axons respond differently to peripheral and central OECs. We then further purified OECs from anatomically distinct regions of the olfactory bulb. Cell behaviour assays revealed that OECs from the olfactory bulb were a functionally heterogeneous population with distinct differences which is consistent with their proposed roles in vivo. We found that the heterogeneity was regulated by motile lamellipodial waves along the shaft of the OECs and that inhibition of lamellipodial wave activity via Mek1 abolished the ability of the cells to distinguish between each other. These results demonstrate that OECs from the olfactory bulb are a heterogeneous population that use lamellipodial waves to regulate cell-cell recognition.